Molecular Cloning, Characterization And Expression Analysis Of Protein Arginine N-methyltransferase 1 Gene(As-PRMT1) From Artemia Sinica

This study firstly cloned PRMT1 gene of Artemia sinica by RACE technology,which got CDNA with the full length 1201 bp which can encod 346 amino acids.Through structure domain analysis,it`s known that the As- PRMT1 contains a PRMT5 combination and a SAM super family structure domains.Multiple sequence alignments revealed that the putative As-PRMT1 protein sequence was relatively conserved across species, especially in the SAM domain. The expression of As-PRMT1 is widely distributed during embryo developemt of A. sinica. This is followed by a dramatic upregulated after diapause termination and is newly downregulation from the nauplius stage. Furthermore, As-PRMT1 transcripts are highly upregulated under conditions of high salinity and low temperature stress. These ndings suggest that As-PRMT1 is stress-related factor and may promote or close the expression of certain genes, play a critical role in embryonic development and resistance low temperature and high salinity stress.