Effect Of Metallic Oxide Nanoparticles On Plant Photosynthesis Mechanism

Natural photosynthesis as earth food supply and the final source of carbon-based fuels and other chemical energy, but also is the key factors of the whole ecosystem balance and the development. In the present posture of the energy shortage and food crisis, photosynthetic cells as a miniature photochemical bioreactor with its tiny precision organizational structure, rapid and efficient conversion of solar energy to attract the attention of mankind. For decades, scientists has developed an amount of artificial materials, including the inorganic semiconductor photocatalytic materials, organic supramolecular photocatalytic simulation system, the biological composite material of artificial photosynthesis systems and the current latest nano bionic technology with the development of nanotechnology and plant engineering, that can efficiently convert the solar energy. These technologies compared to the nature of photosynthesis cells each have advantages and disadvantages, which in turn led to these technologies constantly improve, promote the continuous development of artificial photosynthesis systems.In order to get a efficient reaction system of the conversion of solar energy, and can simply and intuitively study the effects of nanoparticles on the organelles of photosynthesis as well. This paper choose the different plant sources of chloroplasts as the material of restructuring, then established a new photosynthesis reaction system, at the same time detected the photosynthetic activity of thylakoid and the content of metabolic product in the matrix of this system. Restructured reaction systemshowed that the light reaction efficiency of plant chloroplast depends on the photosynthetic activity of thylakoid, and the material exchange between plant chloroplast light and dark reaction part has no difference. Also confirmedthe conclusion that almost no sugar were produced in vitro is correct. It was discovered that the thylakoid membranes in vitro exists with globular form in solution rather than the original layer structure.Otherwise,the aqueous solution of sulfhydrylation nano-materials ?- Fe2O3 nanoparticles, with photocatalytic properties, were added to the restructuring system. By setting different concentrations, photosynthesis activity detection of the system found that ?- Fe2O3 nanoparticles has certain positive effects on the photosynthesis system under the condition of a certain concentration.To further investigate the effect of nano-particles(NPs) on plant photosynthesis mechanism, five typical representative mental oxide NPs are chosen to interacted with the chloroplast which are more stable. The variation of photosynthetic activity and fluorescence effect were measured. Scanning electron microscopy(SEM) and fluorescent microscopy show that the NPs are gathered on chloroplast surface in large quantites. The impact of the result is: the NPs ZnO and CdS has obvious inhibiting effect, and with the increase of concentration the inhibition is more significantly; ?-Fe2O3 NPs can be used as electron acceptor, has some promoting effect at low concentration, but with the increase of the concentration the inhibition effect appeared gradually. Suggesting the impact mechanism of NPs is affect the process of the electron transfer. This work provides a promising approach for the development of the field of NPs in agriculture, including nano-fertilizer, nanobionics and a renewable, high-efficiency biochemical detectors. Meanwhile, it provides a theoretical basis of evaluating the potential effects of NPs on environment.