Study On Function Of The Photoperiod Response Gene StYABBY1 In The Regulation Of Photosynthesis

In the our previous study,the photoperiod response genes,including StYABBYI were identified from potato leaves with different photoperiod treatments by means of transcriptome sequencing.Transcription factor YABBY involves widely in plant organogenesis,but its mechanism on how YABBY determines organogenesis is rarely reported.In this study,transgenic lines,in which expression of the endogenous YABBY1 gene was inhibited was created.Change on the physiological phenotype and proteome of the lines was assayed.The analysis results on assesed data were as follows:(1)A recombinant vector,aiming to inhibit the expression of YABBY1 gene was constructed by insersion of STYABBY1 antisense cDNA into the multiple cloning site of pC1305-35S vector.The recombinant vector was transformed into Agrobaeterium tumefaciens GV3101 and the positive recombinant strain GV3101-pc-35s-StYABBY1 was selected.Through Agrobacterium tumefaciens mediated transformation,Two YABBY1 expression inhibiting lines,yab-11 and yab-14 were obtained by screening on transgenic Nicotiana Benthamiana.(2)The results of morphological observation and physiological phenotype determination showed that there was no difference in morphological phenotype between the control and positive lines at seedling stage,and the seedling height of the positive lines at 60 days was nearly 1/3 higher than that of the control lines.The content of chlorophyll and gibberellin in YABBY1 inhibiting lines was 29%and 22 times higher than that of control lines,respectively,and the net photosynthetic rate of YABBYI inhibiting lines was 4.1 times higher than that of control lines.The results showed that inhibition of YABBYl enhanced chlorophyll synthesis and photosynthesis(3)ITRAQ method was used to determine the proteomic expression profile of YABBY1 inhibited strains and control strains,and t-test analysis was performed to screen the DAPs(differential abundance protein)significantly changing with the relative accumulation amount of YABBY1 decreased.By referring to arabidopsis protein database,the similarity of DAPs and its homologous proteins and the threshold of STRING credibility were set.After STRING correlation analysis of the selected DAPs,it was determined that these DAPs mainly came from chlorophyll synthesis,photophosphorylation and other pathways.(4)GO functional classification and KEGG metabolic pathway enrichment analysis further confirmed that these DAPs were mainly derived from chlorophyll synthesis,carbon fixation,ATP synthesis and other photosynthetic metabolic pathways.The results showed that inhibition of YABBY1 expression increased chlorophyll synthesis and accumulation,enhanced photosynthetic metabolism and increased photosynthetic rate.Thus,StYABBY1 has the function of reverse regulating chlorophyll accumulation and photosynthesis.