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Study On The Immobilized Double Enzyme Catalytic Esterification Of Oleic Acid In Reverse Micelle

Posted on:2016-04-17Degree:MasterType:Thesis
Country:ChinaCandidate:S BaoFull Text:PDF
GTID:2310330470984337Subject:Environmental Engineering
Abstract/Summary:PDF Full Text Request
The lipase and cellulose enzyme immobilization technology and the application of immobilized enzyme were studied. Through the study of single fixed lipase and cellulose effect on lipase activity, the appropriate carrier biochar was chose to be co-immobilized with lipase and cellulose, the technical process of tital fixed to exploe, and to make a preliminary study of the application of immobilized enzyme.The biochar, gelatin and activated carbon were compared as the supports for immobilizing lipase. The immobilization conditions, such as temperature and time were optimized, and then the stability and enzymatic properties of free lipase and immobilized lipase was compared. The results showed that the lipase immobilized on biochar exhibited higher activity and better stability.Cellulase effect on lipase was studied, the activity of lipase was found to be increased by cellulose under optimal treatment conditions. The optimum pH of the cellulose-treated lipase was kept unchanged, while the range was to be wider.Cellulose was chose to be co-immobilized with lipase on biochar. The co-immobilization conditions were optimized. The thermal stability and pH stability of cellulose were improved by immobilization, while the optimal reaction pH value showed unchanged.The rhamnolipid was used to build the RL/isooctane/n-hexanol reverse micelle system. The esterification of oleic acid catalyzed by co-immobilized dual enzymes the rhamnolipid was used to build the RL/isooctane/n-hexanol reverse micelle system. The optimum conditions of esterification by co-immobilized double enzymes were pH of 6.0, RL of 60 critical micelle concentration (CMC),the reaction time for 2h and temperature of 45?,Km of 26.6, and Vmax of 50 mmol·L-1 ·min-1·mg-1.
Keywords/Search Tags:biochar, cellulose, lipase, reverse micelles, oleic acid, rhamnolipid
PDF Full Text Request
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