The Stability Of Mixed Reverse Micellar System And Catalytic Properties Of Laccase In The System

Laccase is a polyphenol oxidase containing copper and can catalyze oxidation of a variety of polyphenols.Its derivatives are widely used in many fields such as food industry,paper industry,environmental protection.It has great significance and value of the research of enzymatic reactions because of use of space and a huge potential for development.Compared with traditional chemical surfactants,rhamnolipid as an environmentally friendly natural surfactant,resulting in microbial metabolic processes of animals or plants,which have loW toxicity,biodegradable eco-compatibility,eco compatibility and certain micellar catalysis ability,etc.It is significant for promoting the application of reverse micellar enzyme system.However,anionic surfactants-rhamnolipid has a strong electrostatic and hydrophobic interactions with enzyme,reducing its activity and stability in the reversed micelles.To solve this problem,a non-ionic surfactant is usually used to weaken the electrostatic interaction with the hydrophobic reverse micellar system,thus weakening the interaction between the enzyme and surfactant.Research is divided into two parts: the first part is intended to study the stability of the mixed reverse micellar system built by Rhamnolipid / Tween-80-hexanol / isooctane applied to the ternary phase diagrams.The second part study some factors such as the molar content of Tween-80,W0,pH,salt concentration and the type of alcohol on laccase activity.By experiment the following conclusions:(1)Construction of the rhamnolipid / Tween-80 / hexanol / isooctane reverse micelles produced using the stability study the stability of the salt solution and pH of the system,this system of pseudo-ternary phase diagrams affected.(2)Laccase shows excellent catalytic performance when overall surfactant concentration of 0.05 mol / L,the optimal reaction conditions: x(Tween-80)= 30%,W0 = 20,the aqueous phase pH4.5,the aqueous phase concentration of KCl 70 mmol / L,cosurfactant n-hexanol.(3)Rhamnolipid / Tween-80 mixed reverse micellar system as an excellent reaction medium significantly improved laccase catalytic performance,under optimum conditions Laccase activity is higher 1.44 times in the mixed reverse micellar system than that inin the single-phase rhamnolipid reverse micelles and 4.35 times than that in water.Laccase showed super active in the mixed system,which declared that surfactant complex has enormous potential in micellar enzymology.