Digitized Molecular Detection Based On Blu-ray Disc Technology:Enhanced Sensitivity

Among the all disc storage technologys, Blu-ray Disc (BD) Technology has the largest storage capacity and the smallest focus diameter. Beyond the intrinsic capability of storing and archiving high-definition audio-video and high capacity data software, it has been adopted to analytical chemistry and molecular detection field, and has the potential to be the POCT (Point of Care Test) tool with high sensitivity, high throughput and low cost.In this paper, we mainly researched the feasibility and veracity of the digitized molecular detection based on standard Blu-ray drive and data error correction software. We first prepared two kinds of spot arrays with different sizes on the surface of bona fide BDs, which are subsequently read with a standard Blu-ray drive in conjunction with the data error correction software program. The feasibility of quantitative analysis, detection throughput and resolution of Blu-ray system were first evaluated. Then we proposed a simple and effective way to convert BDs to biochip platform and successfully prepared molecular binding assays on BDs which are subsequently read and quantitated with a standard Blu-ray drive in conjunction with the data error correction software program. Five conclusions were listed as follows:1. Based on the hydrolysis of lactone, we successfully made the surface of BD more hydrophilic, water contact angle was reduced from 98° to 53° and carboxyl group was created on it.2. The lateral resolution of Blu-ray system can reach 50 μm, the detection throughput can be 280.3. Under Long Distance Code (LDC) and Burst Indicating Subcode (BIS) error correction modes, both LDC counts and BIS counts are proportional to the area of the ink dot, and under the LDC error correction mode, the signal to noise ratio (SNR) is higher. However, the LDC counts reach saturation easily.4. The BIS density (BIS counts per unit assay area) rises proportionally at low concentrations (< 0.5μg/mL), and remains unchanged at high concentrations and it is proportional to optical gray value of binding assays on a large scale.5. The Blu-ray system showed an unpreceded limit of detection (LOD) and sensitivity with respect to previously developed digitized detection methodology which is based on DVD technology.