Study On Femtosecond Laser Multi-function Microscopy Manipulation System

Ultrahigh peak intensity can be achieved at low pulse energy of femtosecond(fs) laser.Three dimensional microfabrication with high precision can be achieved by the ablation of solid material and two-photon photopolymerizatin(TPP) of photosensitive resin.Fs laser micro-and nano-fabrication is a focus in fs laser field.In this paper,we have set up our own microscopy femtosecond laser multi-function operation of the experimental devicewith a femtosecond laser micro-machining,optical tweezers and knife function.Firstly,two photon absorption(TPA) photopolymerization was achieved in ORMOCER resin,the resolution reached 0.7μm,less than the diffraction limitation. At the same time three-dimensional femtosecond laser technology in micro-processing of the material on the two-photon absorption,and optical absorption mechanism of the study and derived two-photon photopolymerization Que value of the mathematical expression on the scanning speed V and the horizontal laser power P Size of the law,and based on this,using two-photon femtosecond laser micro-processing technology to prepare a typical microbial devices-Well arrays and micro-micro-array.Secondly,we use this micro-operation of our devices for optical tweezers on red cell research.Optical tweezers is a three dimensional gradient optical potential trap induced by the interaction of light and substance which represents mechanics effects of momentum transfer.In addition to manipulating micro and nano particles, femtosecond optical tweezers can also induce the nonlinear phenomenon on targets at the same time.With the further development of the research,different kinds of femtosecond optical tweezers appeared.However,there are a few researches on the behavior control.In this paper,the behavior control of red blood cells using femtosecond optical tweezers was investigated by changing the average power of femtosecond laser.The inevitability of steady edging capture of red blood cells was analyzed based on the different relative position between the laser focus and the red blood cell at lower laser power.Nonreversible overturn of red blood cell took place at higher laser power because of the inconsistent gradient force distribution between lateral force and axis force of the femtosecond optical tweezers.Meanwhile,steady transport of red blood cell was achieved by scanning the laser focus.Finally,we operate through this micro-knife device to carry out research on the cells.Based on plasma-induced ablation of cells by fs laser,protuberances of SH-SY5Y cells were successfully dissected.And it finds that the farside the dissection location is,the smaller the dissection effect is.Under un-intact dissection,the longer the dissection length is,the larger the effect is;but the self-recovery of un-intactly dissected cell started earlier than intactly dissected ones.It proved that this fs laser micro-surgery facilitated the research on human nerve’s recovery.