The Method And Research Of Sperm Image Sequence Ultra-long Tracking

WHO laboratory manual for the examination and processing of human semen(5thedition) point out that the longest track time of single sperm is15seconds.This subjectmainly research the sperm sequence image super long time tracking method,for a singlesperm for15seconds long time tracking,the kinematic parameters of sperm of defferenttimes is given,the sperm quality can be a more accurate estimate,apply to the actual testsystem of Nanchang Xierdaier Medical technology company.Adopted image detectionand tracking thought based on small window,for each frame image as long as thedetection and tracking of sperm targets within a small window.The main researchcontent is as follows.(1)Sperm maximum distance prediction is proposed to sovle the problem of asingle sperm long time tracking.Because sperm motion displacement continuitybetween frames,the maximal displacement and direction has a certain limit betweenframes,Based on the these characteristics of sperm movement,vector tracking motionmodel is established.(2)According to the characteristics of sperm morphology to removeimpurities.Normal sperm head area,perimeter and ovality has fixed scope,and betweenthe impurities such as bubbles,while bllod cells,small particles and normal sperm havesignificant differences in morphology,according to these differences by adopting theidea of support vector machine(SVM),the objects are divided into two types,so as toremove impurities.(3)Sperm will encounter motion sperm and immobile sperm disturbances in theprocess of super long time tracking.By detecting the sperm of follow-up frame in thecurrent window,judge interfere with sperm motility.For motility sperm,using themaximum distance prediction to prodict the position in the next frame,match in spermand prediction point nearest to track sperm,which excludes the motion sperminterference.(4)Sperm interference elimination process were studied to solve the fixed sperminterference.As a result of the position of the motionless sperm basically remainunchanged,detect the fixed sperm position in the subsequent frames of the currentwindow,match its nearest to interfere with interfere,so as to exclude the interference. (5)Long time tracking test system is designed,the system contains spermdetection,data analysis,data query and management,and sperm trajectory real-timeplayback,and other functiond, the kinematics parameters of every second and zero toevery second of a single sperm is given.It provides a test tool for setting the standard forsperm long time tracking and build library.Based on more than2000sperm movement types of tracking experiments showthat,the maximum distance method can satisfy the system real time request,and thetracking accuracy of more than97%,for sperm super long time tracking provides basicdata and the realization method directly.