Downregulation Of MiR-18a Or MiR-328 Inhibits The Invasion And Migration Of Lung Adenocarcinoma A549 Cells

【Objective】Lung cancer, which is the malignant cancer with the highest incidence and mortality for men, and ranks second only to breast cancer for women, is a health threat to people. Along with the further research of tumor-associated molecular mechanisms and molecular targeting drug, the patient individualized multidisciplinary comprehensive treatment based on gene targeting will play an more important role.Micro RNA(mi RNA) is a class of 22 nucleotides conserved non-coding small molecule RNA, regulating the target gene expression by affecting tumor cell proliferation, differentiation and apoptosis. This study was to analyze the expression of mi R-18 a and mi R-328 in lung adenocarcinoma A549 cells, and to explore the effect of mi R-18 a or mi R-328 down-regulation on the invasion and migration of A549 cells.【Methods】(1) The relative expression of mi R-18 a and mi R-328 in lung adenocarcinoma A549 cells and normal human bronchial epithelial BEAS-2B cells were detected by q RT-PCR.(2) By obtaining mi R-18 a, mi R-328 sequence, designing and synthesizing the inhibitor sequence and NC sequence, using liposome transfection technology to downregulate the expression of mi R-18 a or mi R-328, the model of A549 cells with low mi R-18 a or mi R-328 expression was established.(3) Further, the Transwell TM invasion and migration assay respectively to detect changes in the groups of A549 cell invasion and migration ability.【Results】(1) q RT-PCR results showed that compared with normal human bronchial epithelial BEAS-2B cells, mi R-18 a and mi R-328 were highly expressed in lung adenocarcinoma A549 cells, and the differences were statistically significant(all P<0.05).(2) q RT-PCR results showed that in lung adenocarcinoma A549 cells and Normal human bronchial epithelial BEAS-2B cells, compared with the blank control group and negative control group, mi R-18 a expression was significantly reduced in transfection mi R-18 a inhibitor group, and the differences were statistically significant(all P <0.05); compared with the blank control group and negative control group,mi R-328 expression was significantly reduced in transfection mi R-328 inhibitor group, and the differences were statistically significant(all P <0.05).(3) Transwell TM invasion experiment results showed that compared with the blank control group and negative control group, transfection mi R-18 a inhibitor group of A549 cell invasion ability decreased significantly, and the differences were statistically significant(all P <0.01); Compared with the blank control group and negative control group, transfection mi R-328 inhibitor group of A549 cell invasion ability decreased significantly, and the differences were statistically significant(all P <0.01).(4) Transwell TM migration experiment results showed that compared with the blank control group and negative control group, transfection mi R-18 a inhibitor group or mi R-328 inhibitor group of A549 cell invasion ability decreased significantly, and the differences were statistically significant(all P <0.01).【Conclusions】In A549 cells, mi R-18 a and mi R-328 showed high expression levels;Downregulation of the expression levels of mi R-18 a or mi R-328 in A549 cells can effectively inhibit cell invasion and migration. Because of its effective inhibiting tumor cell invasion and metastatic ability, mi R-18 a and mi R-328 may become a new target for the treatment of lung malignant tumor.