Effect Of Grape Seed Procyanidins On Invasion And Migration Of Lung Cancer A549 Cells And Its Mechanism

Objective: To explore the effect and mechanism of grape seed proanthocyanidins(GSPs) on migration and invasion of A549 cells.Methods:Trypan blue dye exclusion assay was used to determine the cytotoxic effect of varying dosages of GSPs on the BEAS-2B normal human pulmonary epithelial cells. the proliferation of A549 cells was detected by MTT assay. The migration and invasion of A549 cells were determined by wound scratch assay and Transwell? assay respectively.The expression of EGFR, E-cadherin, N-cadherin in A549 treated by GSPs were detected by Western blotting.Results: 0-40 μg/mL GSPs were not significantly toxic to BEAS-2B cells, but 80 μg/mL GSPs had significant cytotoxic to BEAS-2B cells. The proliferation of A549 cells could be significantly inhibited within limited dosage, the abilities of invasion and migration of A549 cells were also inhibited. The expression of EGFR and N-cadherin were decreased but E-cadherin increased after GSPs-treatment.Conclusions: Grape seed proanthocyanidins(GSPs) is less toxic to normal cells.GSPs can inhibit the abilities of proliferation, migration and invasion of A549 cells that might be related to regulate the expression of EGFR, N-cadherin and E-cadherin.