Study Of IL-4 And IFN-γ Gene Single Nucleotide Polymorphism In Urgur Patients With Pigeon Breeder’s Lung

Objective To study of IL-4 gene and IFN-γ Single Nucleotide Polymorphism in Urgur patients with pigeon breeder’s lung.Methods Clinical data and blood samples were collected in accordance with inclusion and exclusion criteria. A total of 92 cases were divided into the sick group of 32 cases, the unaffected group of 30 cases, and the normal group of 30 cases. DNA was isolated from blood samples. Primer design, sample preparation, PCR, SAP and single base extension were performed by using MassARRAY technology according to SNP loci appendix sequence information, SNPs at the genes for IL-4 and IFN-γ were analyzed using mass spectrometry. Statistical analyses were performed with SPSS17.0 statistical software, genotypes and allele frequencies at each SNP were compared by using the chi-square test.,and calculate the risks associated with the value of OR.Result IFN-γ(rs2069705) genes included of CC, CT, TT three genotypes, IL-4(rs2070874) genes included of CC, CT, TT three genotypes. Three kinds of IL-4(rs2070874) genotypes in the sick group, unaffected group, normal group is no statistical difference in the distribution of frequency, and the C, T allele frequency in each group showed no significant difference(χ2=2.607,P=0.626;χ2=2.244,P=0.326), the genotype frequency between each two groups also showed no significant difference. The three kinds of IL-4(rs2070874) genotypes in the sick group, unaffected group, normal group is no statistical difference in the distribution of frequency, and the C, T allele frequency in each group showed no significant difference(χ2=6.614,P=0.158;χ2=5.408,P=0.067),but genotype frequencies between each two groups showed differences were statistically significant in the sick group and unaffected group.(χ2=6.67, P=0.036). The TT homozygotes of sick group compared with the number of CC homozygotes and heterozygotes of CT, TT homozygotes occurrence of PBL OR(95% CI) were 0.11(0.02 ~ 0.68), 0.16(0.03 ~ 0.79), P values were 0.018,0.025, the difference was statistically significant. The difference between CC homozygotes and heterozygotes CT was not statistically significant(P=0.402;P=0.134).Conclusion 1.IFN-γ(rs2069705) polymorphism may be involved in the development of Uighur PBL, but it may not play a role in the pathogenesis of PBL, which need increase the sample size to further confirmed. 2.IL-4(rs2070874) polymorphisms may be involved in the occurrence and development of Uighur Pigeon Breeder’s Lung(PBL).3. IL-4(rs2070874) carrying TT homozygotes may be a risk factor for susceptibility of Uighur PBL, and may increase the risk of PBL, and need increase the sample size to further validation.