The Expressive Differentiation Of SLP-2 In Human Placenta And Its Role In Pregnancy Outcomes

Objective:Normal placentation and successful pregnancy are partially determined by well-controlled trophoblast differenciation, migration and invasion into the endometrium and one third of the myometrium, as well as the associated spiral arteries. Our previous study has revealed that SLP-2, also known as stomatin like protein 2, was over-expressed in human early placenta, whereas whose roles in pregnancy outcome remain unknown. In this essay, we performed IHC and WB to explore the expressive differentiation of SLP-2 in human placenta and provide theoretical basis to its role in pregnancy outcomes.Methods:The expression of SLP-2 in human first and third trimester placenta was determined using immunehistochemistry. Western blot was used to identify the difference of SLP-2 expression level among normal villi, embryo damage(missed abortion) villi, normal labor placenta, cesarean placenta and placenta from woman suffered from severe-preeclampsia and underwent cesarean.Results:SLP-2 was strongly expressed in syncytiotrophoblasts and trophoblast columns of human placental villi, extravillous trophoblast cells and glandular epithelium within the maternal decidua. The occurrence of embryo damage during the first trimester and severe-preeclampsia has a significant correlation with the down-regulated expression of SLP-2.Conclusion:These findings strongly suggest that SLP-2 plays an important role in human placental development. Abnormal SLP-2 expression could have a relationship with adverse pregnancy outcomes such as embryo damage during the first trimester and severe-preeclampsia.