Expression Of Rce1 In Prostate Cancer And Its Clinical Significance

Objective: Rce1(Ras and a-factor converting enzyme 1) have been reported to play a key role in the proteolytic processing of Ras protein. The present study aimed to investigate the expression and prognostic significance of Rce1 in patients with PCa.Methods: The mRNA and protein expression levels of Rce1 were analyzed in 12 pairs of PCa and BPH specimens by q RT-PCR and Western blotting, respectively. Immunohistochemistry(IHC) was performed to examine protein expression and location of Rce1 in 74 archived PCa tissues and 30 BPH tissues. The association between Rce1 expression and the specific clinicopathologic features was evaluated by χ2 tests. Kaplan-Meier and Cox proportional hazards regression models were used to analyze the data.Results: Expression of Rce1 was markedly higher in 12 PCa specimens than that in the paired BPH specimens at the mRNA and protein levels. Moreover, Expression of Rce1 in PCa was strongly associated with clinicopathologic features. It was detected in 69(93.24%) of 74 PCa tissues by immunohistochemistry, and it was found to be associated with Gleason score(P = 0.013), T class(P = 0.015), and distant metastasis(P = 0.044). Patients with PCa having higher Rce1 expression had substantially shorter survival times than patients with lower Rce1 expression. Univariate and multivariate analysis revealed that Rce1 was an independent prognostic factor.Conclusion: Our study suggests that expression of Rce1 can serve as an independent biomarker for the prognosis of PCa patients.