Expression Of Rce 1 In Baldder Cancer And It’s Influence On Cell Cycle And Apoptosis Of T24 Cells

Objective: To investigate the expression and clinical significance of Rce1(Ras and a-factor converting enzyme 1) in tissues of bladder urothelial carcinoma(UHC)and pericarcinoma(PC); To explore the effect of lentivirus-mediated shRNA silencing Rce1 gene on apoptosis and cell cycle of human bladder cancer T24 cells.Methods: Immunohistochemical staining for Rce1 expression was performed in bladder urothelial carcinoma( UCB) and adjacent pericarcinoma tissues;the methods of quantitative real-time polymerase chain reaction( qRT-PCR) and western blotting were utilized to investigate mRNA/ protein expression of Rce 1 in UCB;Rce1(+)and Rce1(-) patients were compared in terms of pTNM grade and differentiation grade. Lentiviral interference vectors of sh RNA-Rce1 were constructed successfully and were transfected into T24 cells, then the T24 cells stably knockdown Rce1 gene expression were screened by puromycin, Expression of Rce1 mRNA and protein in T24 cells was detected by RT-PCR and western blot,respectively. Apoptosis of T24 cells were examined by flow cytometry.Results: The expression of Rce1 were highly up-regulated in both mRNA and protein level in carcinoma tissues when compared with the adjacent ones through qRT-PCR and western blot analysis. By IHC, positive expression of Rce1 was examined in 55/78(70,5%) of UCBs and in 4/21(19.0%) of paired pericarcinoma tissues. Positive expression of Rce1 was correlated with poorer differentiation, higher T classification and tumor metastasis(P < 0.05). In Kaplan-Meier survival analysis, a significant association between positive expression of Rce1 and shortened patients’ survival was found(P < 0.001). Compared with those in control group and negative control group,expressions of Rce1 m RNA and protein were significantly reduced after Rce1-shRNA lentiviral interference vectors being infected(P=0.00),apoptosis was increased significantly(P=0.000).Conclusions: The expression of Rce1 is significantly higher in UCBs when compared to pericarcinoma tissues, positive expression of Rce1 also correlated with prognosis of patients with UCB; lentivirus-mediated shRNA silencing Rce1 gene can down-regulate the expression of Rce1 gene, promote apoptosis in T24 cells, Rce1 may serve as a biomarker of diagnosis and prognosis in patients with UCB.