Genetic Polymorphism Study Of Y-SNP In Yunnan Wa Male Population

[Objective] This topic on the basis of the Y chromosome system evolutionary tree, select all of the trunk on 35 Y-SNP sites, from the perspective of paternal inheritance, to the Y-SNP genetic markers were analyzed, and the purpose is to build on the basis of the Y-SNP DNA database, laying the groundwork for a forensic application, and carries on the comparison to related population genetic relations, to further explore the source of the wa and relations with other nations.[Methods] In line with the principles of voluntary informed consent, in strict accordance with the criteria (male unrelated individuals, within three generations are wa, unrelated), in yunnan province to collect 276 cases of peripheral venous blood of 5 ml, wa men EDTA-Na2 anticoagulation, and test sample Y chromosome MassARRAY method system evolutionary tree 35 Y-SNP loci, the MassARRAY technology to detect genetic polymorphism, SNP loci of the application of SPSS 19.0 statistical software for statistical analysis.[Results] 276 cases of male crowd 35 Y Yunnan Va Y-SNP haploid type frequency, In selected 12 backbones in the haploid group, there are 7 mutations, the largest number O, K, N mutations, accounted for 31.87%,29.35%and 29.35%respectively; Based on the Y-SNP haploid group D, Dl, C, F, G, N, O, P, the frequency of the evolutionary tree of the prototype system, clustering analysis and principal component in the two-dimensional analysis diagram, cluster class conforms to the characteristics of east Asian populations.[Conclusions] This topic based on the tree of human Y chromosome system, analysis of yunnan wa Y-SNP loci,276 cases male sample were tested the 35 Y-SNP loci, the Va people in yunnan haploid type frequency and genetic structure information, in order to establish yunnan Va men crowd-based DNA SNP database provides data reference.