| Diarrhea is one of the common livestock dieases, it is caused by gastrointestinal function and bacteria or virus infection. Sanguisorba and serchemia sinica are traditional Chinese medicine which have the anti-diarrhea effect. The diarrhea model of mice induced by folium sennae and the intestines push movement model were used to study the anti-diarrhea effect about compound sanguisorba and berchemia and the mechanism of aquaporin, aiming to explore the treatment of diarrhea of the compound and provide experimental bases for its application in the veterinary clinical in the future.The mice diarrhea model was made by gavage at dose of 0.2 g/kg folium sennae apozem, the intestines push movement model and excited intestine push movement model were created by gavage with carbon powder and the by intraperitoneal injection with neostigmine methylsulfate respectively. The experiment groups were divided into saline control, high, middle and low dose of compound sanguisorba and berchemia groups (1.0 g/kg,0.5 g/kg,0.25 g/kg), compound diphenoxylate as the positive control, the neostigmine model control was added in intestinal propulsive tests. The diarrhea frequency and rate of dilute feces were used to evaluate the diarrheal influence caused by folium sennae, the HE staining was used to observe the villus’ change in the colon. Intestinal propulsion rate was used to evaluate the intestines push movement’s influence about the compound. Results showed the diarrhea frequency and rate of dilute feces in three doses of tested compound and positive control were clearly lower than that of saline control at 4-6 h post administration(P< 0.05). No obvious change on the colon’s villus in all groups were found based on the HE staining analysis. The intestinal propulsion rate in 1.0 g/kg,0.5 g/kg and 0.25 g/kg tested groups and positive control were significantly lower than that of normal control (P<0.05). The intestinal propulsion rate in 1.0 g/kg,0.5 g/kg and 0.25 g/kg tested groups and positive control were significantly lower than that of model control by neostigmine, and there were no difference between three tested groups and the saline control (P> 0.05). The compound sanguisorba and berchemia tested appeared diarrhea inhibition at doses of 0.5-1.0 g/kg and intestinal propulsion restraint at doses of 0.25-1.0 g/kg on normal or intestinal excitation mice.In mice diarhea model caused by folium sennae, the change of AQP4 in the proximal colon by Western Blot and IHC were made in 0.5 g/kg of the compound, folium sennae control group and saline control group for 2 h,4 h and 6 h administration. The AQP3 mRNA in distal colon and AQP4 mRNA in the proximal colon were tested by RT-PCR. Results showed that the expression of AQP4 at 2 h,6 h in the folium sennae and 2 h in 0.5 g/kg group were extremely significantly lower than that of saline control (P<0.01), and that of 4h treated by 0.5 g/kg group was significantly lower than saline control (P<0.05). There was no significant difference in 2 h of the folium sennae,2 h of 0.5 g/kg group and saline control (P>0.05). The level of AQP4 at 6 h 0.5 g/kg group was significant higher than that of folium sennae 6 h (P< 0.05). Results of IHC tests showed the AQP4 in the on colon villus in 0.5 g/kg group was obvious increased compared to folium sennae group, but it could not exceed saline control group. The expression of AQP3 mRNA in folium sennae at 4 h and 6 h were extremely significantly lower than that of saline control (P<0.01), there were no obvious difference between the 2 h,6 h of 0.5 g/kg group, folium sennae 4 h and saline control group (P>0.05). The level of AQP3 mRNA at 4 h of 0.5 g/kg group was higher than the 4 h of folium sennae group, but no significant difference (P> 0.05). The level of AQP3 mRNA at 6 h of 0.5 g/kg group was extremely significantly higher than that of 6 h of folium sennae (P<0.01). The changes of AQP4 mRNA in different groups were same as the AQP3 mRNA. The expression of AQP4 and the mRNA of AQP4 and AQP3 were significantly decreased in diarrhea caused by folium sennae, the compound sanguisorba and berchemia can resist the decrease of AQP3 and AQP4 induced by diarrhea.In conclusion, the compound sanguisorba and berchemia appeared obvious inhibitory effect on the diarrhea caused by folium sennae and the excited intestine caused by neostigmine methylsulfate, resisting the decrease of AQP3 and AQP4 in the diarrhea model. So, it would be hopeful to be used in the treatment of the diarrhea caused by unbalance of intestinal function. |
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