The Construction Of Differentially Expressed Genes Spectrum Of Parotid Gland Tissue In Rats After Radiation And Bioinformatics Analysis

Objective:To analysis the differentially expressed genes spectrum of parotid gland tissue in rats after radiation using whole genome microarrays, to screen the important genes and pathways by using related bioinformatics.Methods:Twelve SD rats were randomly assigned to vehicle and radiation groups of six animals each.15Gy 60Co-γ ray was used in radiation group, and the rats in vehicle group were injected intraperitoneally with 10% chloral hydrate. After 2 hours’ irradiation, the parotid gland tissue was frozen in liquid nitrogen until analysis. Total RNA of the extracted parotid tissues are perform reaction of Cye Dye Labeling fluorescence labeling and Phalanx OneArray TM hybridization. ROAv2 Rat OoeArray(?) including 20717 genes was used to screen the whole genome microarrays, using the|log2 (ratio)|≥1 as the cutoff threshold. GO functional classification and pathway analysis were performed on screened differentially expressed genes.Results:After clustering analysis,913 differentially expressed genes were obtained, including 455 up-regulated genes, and 458 down-regulated genes.913 differentially expressed genes were divided into 13 major functional genes categories using GO functional classification, mainly related to metabolism, transcription regulation, cell death, etc. Pathway analysis showed 13 pathway changes after radiation, including 75 expressed genes. In addition, the following 7 genes were enriched in 4 or more pathway changes:Akt1, Pik3rl, Pik3cg, Nfkbia, Kras, Mapk13, and Casp8.Conclusion:Significant changes were found in the expression of genes spectrum of parotid gland tissue in rats after 60Co-y radiation, such as Aktl, Pik3rl, Pik3cg, Nfkbia, Kras, Mapk13, and Casp8. These genes may be involved in the process of carcinogenesis of post radiation parotid gland.