The Expression And Clinical Significance Of Apoptosis’s Related Gene Bcl-2、Bax And NF-KB In Adenoid Cystic Carcinoma Of The Parotid Gland

Objective:To observe the expression and correlation of Bcl-2, Bax and NF-KB in the parotid ACC, explore the occurrence,development and prognosis of biological significance with ACC of the parotid gland.Methods:Detected the expression of Bcl-2, Bax and NF-KB gene protein in49cases of ACC wax block embedded tissue in Immunohistochemistry SP method,20cases of normal parotid tissue(NP) as control. Using x2test, the statistically significant difference is defined as p<0.05.Results:l.The total expression rate of Bcl-2in ACC group is67.35%(33/49), significantly higher than NP group (35.00%7/20)(p<0.05);The expression of Bcl-2in different clinical TNM stages and lymph node metastasis of ACC are difference (p<0.05);the expression of different pathological typing is no differences (p>0.05).2.The group’s total expression rate of Bax in ACC is32.65%(16/49), higher than NP group (20.00%4/20),but there was no difference (p>0.05);The expression of different pathological type, different clinical TNM stage, lymph node metastasis was no difference in ACC (p>0.05).3.The total expression rate of NF-KB in ACC group is69.39%(34/49), which is significantly higher than the rate of NP group (35.0%7/20)(p<0.05); The expression of Bcl-2in different clinical TNM stages and lymph node metastasis of ACC are difference (p<0.05);the expression of different pathological typing is no differences (p>0.05).4.There is negative correlation trend between the Bcl-2and Bax (kappa=-0.057),but no difference in the statistics (p>0.05).5. There ia some positive correlation between Bcl-2and NF-KB (p<0.01kappa=0.387). Conclusion:1. Bcl-2and NF-KB have close relation with the occurrence, development arid prognosis.They can be used as the important indicators of the occurrence,development and prognosis of ACC of the parotid giand.2. NF-KB plays a important role by up-regulating the expression of Bcl-2in the occurrence and development process of adenoid cystic carcinoma of parotid gland.3.The expression of Bax is positive in ACC of the parotid giand, But there was no difference in the statistics. There are no relationship between pathological type, clinical stage and lymph node metastasis.