| Objective:protective effect of research and of Zaojiaodahuang decoction (ZDD) on CC14 induced in mice with acute liver injury and to explore its possible mechanism.Methods:1.60 Kunming male mice under normal feeding condition were divided into 6 groups according to the complete random grouping method, the normal control group, the CC14 model group and the positive control group, respectively. ZDD of high, medium and low dose group, with 10 mice in each group.2. Normal control group and CC14 model group were given daily volume of sterile saline, the positive control group was given 0.10mg/kg. ZDD of high, medium and low dose group dosage. respectively:2. OOg/kg,1. OOg/kg,0.50g/kg, delivery volume is 0.2ml/10g. Administration method was administered once a day for 7 days.3. The experiment began to make the model before blood collection mice 24 hours fasting can not help but water. After the model was made, the eye blood was taken out, and the blood was taken out of the eyeball. After the mice were killed by cervical dislocation. The mice were sacrificed by laparotomy, and then took out about 0.1g liver tissue, and then added 0.9mL saline to the homogenate.4. The final determination of serum Alanine Aminotransferase (ALT), the Aspartic the Amino transaminase (AST) activity, the Glutathione peroxidase (GSH-PX), the activity of total superoxide dismutase (T-SOD), the content of liver homogenate malondialdehyde (MDA)Results:Zaojiaodahuang decoction high, low dose group and CC14 model group compared to serum alanine ALT, AST content decreased significantly; significantly increased the activity of total SOD and GSH-Px, liver tissue MDA content decreased significantly.Conclusions:This research confirmed the protective effect of ZDD on acute liver and drug injury caused by CC14. The mechanism may be related to scavenging oxygen free radicals and inhibiting lipid peroxidation. |
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