Studies On Protection Effect And Mechanism Of Soyasaponins On Mice With Subacute Hepatic Injury

Objective:To protective effect and mechanisms of soyasaponins on Mice with subacute hepatic injury by CCl4.Methods:Mice were randomly divided into normal control group, model group, soyasaponins group including low、medium and high medication (50、100、200mg/kg), and silymarin group (100mg/kg); normal group and model group were fed with saline daily20mg/kg everyday for28days, gavage for28days after the administration8h,in addition to the normal group,others groups intraperitoneal injection of CCl4soybean oil solution32mg/kg every three days, the last administration8h,fasting but water after16h to obtain blood and liver. The liver used to observe the histopathological changes in the liver sections stained with H&E were examined; Colorimetric method assay the activity of the mice serum ALT and AST; Examination of liver homogenate MDA and GSH levels and activities of the antioxidant enzymes SOD, GST, CAT and GSH-PX; TNF-α、IL-1β、 NO and PEG2in the serum was detected by the ELISA method.We ues Western Blot method to detect liver tissue in the cytoplasm COX-2and iNOS, HO-1the protein expression of therapeutic targets, detection of cytoplasm protein ERK,p38,JNK and its phosphorylated proteins expression, detection of nucleus protein NF-κB and its phosphorylated proteins expression, detection of apoptotic protein caspase3,Bcl-2, Bax expression.Results:1. Compared with the normal control group,CCl4model group the activity of ALT and AST in serum increased(P<0.01), compared with the CCl4model group, soyasaponins group serum ALT and AST activity were significantly decreased (P<0.01, P<0.05).2.The morphological results showed that soyasaponins attenuated liver histopathological changes induced by CCl4in mice.3.Compared with the normal control group,CCl4model group the content of MDA in liver homogenate increased (P<0.01), but the GSH content and CAT, SOD,GSH, GSH-PX and GST activity decreased (P<0.01), compared with the CCl4model group,soyasaponins group MDA content decreased (P<0.01), GSH content increased (P<0.01), CAT, SOD, GSH and GSH-PX and GST activity increased (P<0.05, P<0.01, P<0.05, P<0.01).4.Compared with normal group,CCl4model group serum the content of TNF-α、IL-β、NO and PEG2increased, compared with the CCl4model group, soyasaponins group serum the content of TNF-α、 IL-β、NO and PEG2were significantly decreased.5.Western Blot to detect the protein the expression of important therapeutic targets, compared with normal control group, CCl4model group HO-1, COX-2and iNOS protein expression increased, compared with CCl4model group,soyasaponins group HO-1, COX-2and iNOS protein expression decreased; detection of upstream signal path NF-κB, ERK, p38, JNK and its phosphorylated proteins expression, compared with normal control group, CCl4model group NF-κB, ERK, p38, JNK and its phosphorylated proteins expression increased, compared with CC14model group,soyasaponins group NF-κB, ERK, p38, JNK and its phosphorylated proteins expression decreased;detection of apoptotic protein caspase3, Bcl-2, Bax expression, compared with normal control group, CCl4model group caspase3and Bax protein expression increased, Bcl-2protein expression decreased, compared with CCl4model group,soyasaponins group caspase3and Bax protein expression decreased, Bcl-2protein expression increased.Conclusion:Soysaponins has a protective effect on the mice with subacute hepatic injury induced by CC14, the protection mechanism may be related to inhibition of lipid peroxidation and enhance antioxidant capacity of liver, inhibit inflammatory reaction and inhibit liver cell apoptosis. Its anti-inflammatory effect is in connectiong with the NF-κB and the adjustment of MAPK signal pathway.