Mechanism Of Curcuma Aromatica Against Acute Liver Injury Induced By Carbon Tetrachloride

Curcuma aromatica is a kind of plant of the genus Curcuma. WarmCurcuma, curcuma and Curcuma kwangsiensis S.Lee et C.F.Liang or The dried tuberous root of Epicurzerenone have a long medicinal history. Modern research turns out that Curcuma aromatica Salisb have immunity of repress, cholesterol-lowering, the anti-oxidize, anti- fungi, anti- tumor, protecting liver and repressing axis etc. various pharmacology activities. It can be used for angina pectoris, hepatitis, old age stupid, malignant tumor etc. disease. However, the effect and mechanism of Curcuma aromatica aginst liver injuries has not been clarified. We have studied the effect and mechanism of Curcuma aromatica against liver injuries, in order to elucidate new pharmacological action and new medicinal value, and furnish the theoretical basis for developing and exploiting new drug from Curcuma aromatica against liver injuries .Objective :Mechanism of Curcuma aromatica against acute liver injury induced by Carbon tetrachlorideMethods :The experimental, male and female with equal quantity,48 animals, cleaned by ICR, were randomly divided into 6 groups by weight:control group ,model group ,drug positive group(Ganlixin),Curcumae group high, middle, low groups (l2g/kg, 6g/kg, 3g/kg).The first group was not treated with CC14 treatment and served as the control.The model mice of acute hepatic injury were set up by giving intraperitoneal injection (25mg/kg-1) of 0.2% CC14(0.2m1/lOmg). Ganlixin group(25mg/kg-1)and there three therapeutic groups of Curcumae which were intragastric at the doses of 3g/kg-1,6g/kg-1,l2g/kg-lonce everyday. 7 days later all mice were put to death. A soma piece of liver was taken O.5cm far from the edge of lobes of liver,put into the 10% formal fixed for pathologic test another . The index of the liver was calculated ;The spleen lymphocyte proliferation induced by ConA were checked with MTT;Pathological change was examined by HE staining method .The gene expressions of IL-1β,TNF-a,caspase-3,Bcl2 and Bax were analyzed with RT-PCR. The protein expressions of caspase-3,Bcl2 and Bax were analyzed with immunohistochemistry staining .Results: Compared to normal mice, the index of liver of model group were obviously increased, while the treatment group had a lower level than the model(P﹤0.05),Proliferat ion of spleen lymphocyte induced by CoA could be enchanced(P﹤0.05); At the model group, there were a lot of inflammatory cells infiltration and necrotic hepatocytes in liver, but the lesions became much slighter at the treatment group. Compared with model group, the geneexpression of IL-1βand TNF-a was significantly down-regulated in liver in treatment group (P<0.01). The gene and protein expression of bcl-2 were significantly up-regulated in liver in treatment group (P<0.01),but those of bax and caspase-3 were down-regulated (P<0.01).Conclusion : It turns out from the above words that the Curcuma aromatica has the protective effects to CCI4-induced acute liver injury. Its function mechanism is related to decreasing oxygen free radicals, repression of expression of inflammatory cells, Immunoregulatory and anti- Apoptosis.