| ObjectiveThis study through synthesizing the thinking mode of tutor treat constipation and clinical experience, combined with previous experimental data, tries to from two angles of clinical and experimental research to observe Chua’s spleen laxative decoction" in the treatment of spleen deficiency constipation clinical efficacy and long-term effect.MethodsAnimal experimental research section:Choose 156 kunming mice, male and female half, weighting (±2) 20g, all the mice apply compound factors made spleen deficiency constipation modeling method, they were randomly divided into 6 groups:blank control group (normal group), model group, Chua’s spleen laxative decoction high dose group (high dose group), Chua’s spleen laxative decoction middle dose group (middle dose group), Chua’s spleen laxative decoction low dose group (low dose group) and lactulose group. After modeling was complete, the treated mice were each administered treatment for 7 days to observe the general situation of each group of mice, the changes of body weight and stool. In each group,8 mice were randomly selected to observe the general conditions, body weight and stool of mice after stopping the drug, the remaining mice did the following tests:With ink propulsion test for detection of intestinal propulsion, determination of the serum D-xylose content by colorimetric analysis, observation of intestinal pathogical changes by hematoxylin-eosin (HE) staining.Clinical case study section:42 cases of outpatients and inpatients with constipation due to spleen deficiency from March 2016 to March 2015 in the first Chinese Medicine Hospital of Zhanjiang City were randomly divided into Chua’s spleen laxative decoction (treatment group) and lactulose group (control group),21 cases in each group, respectively, compare clinical efficacy in two groups, the main clinical symptoms and associated symptoms, colonic transit improvement, adverse reactions and withdrawal two months after the recurrence of constipation.ResultsAnimal experimental research section 1. Weight:after treatment and withdrawal after 8 days. Compared with the normal group, model group body weight were decreased significantly (P<0.05); compared with the model group, body weight of mice in each treatment group were significantly increased (P <0.05) and after treatment, the high dose group were significantly increased body weight lower dose group (P<0.05).2. Feces after gavage 6h:Compared with the model group, the treatment groups of mice feces quantity, weight and grain stool weight were increased significantly (P<0.05); compared with the normal group, model group mice grain weight of feces and the amount were decreased significantly (P<0.05); compared with the normal group, the low dose group, the lactulose group and model group mice stool weight were decreased significantly (P<0.05).3. Feces after stopping drug:Compared with the normal group, model group of mice feces number, weight and grain stool weight were decreased significantly (P<0.05); compared with the model group, the treatment groups of mice feces quantity, weight, grain stool weight were increased significantly (P<0.05); compared with the high dose group, the low dose group, the lactulose group grain stool weight were decreased significantly (P<0.05); compared with the medium dose group, the lactulose group and model group grain stool weight were decreased significantly (P <0.05).4. Intestinal propulsive function:Compared with the normal group, model group mice intestinal propulsion rate were significantly lower (P<0.05), compared with the model group, the mice in each treatment group intestinal propulsion rate were significantly higher (P<0.05), especially the high dose group and the middle group intestinal propulsion were significantly higher than low dose group and lactulose group (P<0.05)).5. Serum levels of D-xylose: Compared with the normal group, model group serum D-xylose levels in mice were significantly decreased (P<0.05); compared with the model group, the treatment groups were significantly increased serum D-xylose (P<0.05).Clinical case study section 1.After administration, the total effective (95.24%) in the treatment group was higher than that in the control group (85.71%), but the difference between the two groups was not significant (P>0.05).2. After administration, the main clinical symptoms and symptoms of constipation in the treatment group were significantly lower than those in the control group (P<0.05).3. After administration, the total colonic transit efficiency in the treatment group improved significantly higher (P <0.05).4. Adverse reaction rates during the administration of the treatment group was significantly lower than the control group (P<0.05).5. Treatment of patients with constipation recurrence rate was significantly lower than the control group (P<0.05) after stopping drug 2m.ConclusionChua’s spleen laxative decoction has remarkable effect on spleen deficiency constipation mice, It has the function of invigorating the spleen, particularly in the high and middle dose group, outstanding performance in the improvement of the grain weight of feces after stopping drug and promoting the function of intestinal propulsion; Chua’s spleen laxative decoction on spleen deficiency constipation patients have better clinical efficacy, mainly in taking advantage of the adverse reactions and recurrence after withdrawal, worthy of recommendation. |
PDF Full Text Request