Associationof Selp Polymorphismwith Essential Hypertensionsuscepibility And Interaction With Sele

Objective:In the present study, we aimed to identify the association of single nucleotide polymorphism (SNP) of P selectin (SELP) with Essential Hypertension and investigate the relationship with E selectin (SELE).Methods:The subjects included 407 patients with Essential Hypertension (EH) and 677 EH-free controls in the case-control study. All subjects were interviewed in person. Information on demographic characteristics, smoking status, drinking status and so on was collected by a unified questionnaire. A 3 ml blood sample was drawn to extract genomic DNA in the method of Phenol-Chloroform. The three SNP sites of SELP were genotyped with Matrix assisted laser desorption/ionization time-of-flight mass spectrum (MALDI-TOF-MS) technique. Group-t test and x2 test were used to analysis measurement data and count data, respectively. Odds ratio (OR) and 95% confidence interval (CI) were obtained from unconditional logistic regression model to evaluate associations between SELP polymorphism and susceptibility to EH. The Hardy-Weinberg genetic equilibrium (HWE) was tested by Stata software. LD analysis and haplotype analysis were analyzed with SHEsis software. MDR method was used to test the interaction of SNPs between SELP and SELE. All tests were two-sided and a P value of<0.05 was considered statistically significant.Results:(1) The general characteristics of the subjects:There were no statistical differences in the distributions of sex, BMI, cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein between cases and controls (P>0.05), respectively. The distributions of age and alcohol consumption were obviously difference (P< 0.05).(2) Association between SELP polymorphism and essential hypertension:The genotype distributions of 3 SNPs were in agreement with Hardy-Weinberg Equilibrium in controls (p>0.05). Notably, genotypes (AA、AG、GG) were significant differences between the two groups in the only SNP site rs3917739(P<0.05). As the results of unconditional logistic regression model (Adjusted by age and alcohol consumption) show, who carried AA genotype suffer from a decreased risk of essential hypertension comparing with GG genotype (OR=0.456,95%CI:0.217-0.957, P=0.038). There were no significant associations between other Polymorphism and essential hypertension risk. Further analysis showed that recessive model (AA+AG vs GG) of rs3917739 was associated with EH. After adjusting the age and alcohol consumption, GG genotype increased the risk of EH comparing with AA+AG genotype (OR=0.480,95%CI:0.235-0.978,P=0.043)(3)Haplotype analysis:There was no linkage disequilibrium among the three SNP sites. The results indicated that the haplotype G-C-G of SELP is a susceptible factor of EH (OR=1.487,95%CI:1.032-2.117,P=0.032). The haplotype frequency of in case was significant higher than control.(4)Relationship between SLEP and SELE:The MDR analysis did not observe any significant reaction between SLEP and SELE related to EH.Summary:For rs3917739, AA genotype decreases the risk of essential hypertension, and recessive model (AA+AG vs GG) is associated with essential hypertension; The haplotype G-C-G increases the risk of essential hypertension; The interaction between three SNPs in SELP and rs4786 in SELE is not significant.