| Objective To explore expression level of HMGB1 in glioma tissue and epileptogenic focus tissue around glioma of glioma-related epilepsy patients, and analye its significance in glioma pathological characteristic and preoperative epileptic seizure.Methods 40 glioma-related epilepsy patients who underwent “resection of glioma and epileptogenic focus” in Neurosurgery Department of the Fifth Affiliated Hospital of Zhengzhou University during September 1, 2013 to September 1, 2015 were selected as experimental group. Glioma tissue and epileptogenic focus tissue was taken during operation for experimental detection. Locating epileptogenic focus by intraoperative cortical electroencephalogram(ECo G) monitor. To record clinical information, such as, gender, age, location of glioma and epileptogenic focus, preoperative epilepsy duration, preoperative epilepsy frequence, preoperative epilepsy type, pathological grade and type of glioma tissue. 40 glioma patients who underwent “resection of glioma” in Neurosurgery Department of the Fifth Affiliated Hospital of Zhengzhou University in the same period were selected as positive control group. Glioma tissue was taken during operation for experimental detection. To record clinical information of patients in positive control group, such as, gender, age, location of glioma, pathological grade and type of glioma tissue. 20 patients who underwent “intracranial decompression” in the same hospital, same period were selected as control group. The brain tissue was taken during operation for experimental detection. To detect HMGB1 expression in glioma tissue of experimental group and positive control group, epileptogenic focus tissue of experimental group, and brain tissue of control group via immunohistochemistry and Western blot analysis. Statistically analyzed correlation of HMGB1 expression level in related brain tissue with clinical information, glioma pathology and preoperative epileptic seizure. A value of P<0.05 was considered statistically significant.Results Positive expression rate of HMGB1 in 80 glioma tissue was 100%, which was higher than in brain tissue of control group(25%)(x2=70.588,P=0.000). Positive expression rate of HMGB1 in epileptogenic focus tissue in experimental group was 100%, which was higher than in brain tissue of control group(25%)(x2=40.000,P=0.000). Expression of HMGB1 in glioma tissue of experimental group was not correlated with gender, age, location of tumor, preoperative epilepsy duration, preoperative epilepsy frquence, preoperative epilepsy type, pathological type of glioma tissue. While was positively correlated with pathological grade of glioma(r=0.338,P=0.033). In experimental group, expression of HMGB1 in epileptogenic focus tissue was not correlated with gender, age, location of epileptogenic focus, preoperative epilepsy type, pathological grade and type of glioma, while was positively correlated with preoperative epilepsy duration(r=0.504, P=0.001), preoperative epilepsy frequence(r=0.427,P=0.006). In LGG patients, HMGB1 expression in glioma tissue was correlated with epileptic seizure(x2 =6.539,P=0.011), while in HGG patients, the correlation was not found(x2 =1.027,P=0.311). Multiple factors analysis showed LGG(P=0.024), high expression of HMGB1 in glioma tissue(P=0.000) were independent risk factors to glioma-related epilepsy.Conclusion 1. HMGB1 is overexpressed in glioma tissue, and HMGB1 expression level is positively correlated with pathological grade. HMGB1 might involove in the glioma cells differentiation through mediated inflammatory response and impact pathological grade of glioma tissue. HMGB1 might become a new target to judge pathological grade of glioma tissue and provied a new way to study glioma. 2. HMGB1 is overexpressed in epileptogenic focus tissue of glioma-related epilepsy patients. HMGB1 expression level is positively correlated with preoperative epilepsy duration and frequence. HMGB1 might provide a new way to study and treat glioma-related epilepsy. |
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