Detailed Analysis Of Oxidative Stress Level In IVF-pregnancies With Intrauterine Hematoma In The First Trimester

Objective To investigate oxidative stress level in the in vitro fertilization(IVF)-pregnancies diagnosed with intrauterine hematoma(IUH).Methods A case-controled study of 242 IVF-pregnancies, including 121 women with intrauterine hematomas and 121 controls without who matched for the number of fetuses and way to achieve pregnancy was performed to compare the level of oxidative stress with intrauterine hematomas during the first trimester and controls at the First Affiliated Hospital of Anhui Medical University from July 2014 to September 2015. And according to the number of gestational sac, two groups were further divided into two small groups(singleton pregnancies and twin pregnancies) respectively. Data on maternal characteristics were collected from medical records and through telephone follow-up. ELISA was used to measure the maternal serum reactive oxygen species(ROS), superoxide dismutase(SOD), glutathione peroxidase(GPx) and malondialdehyde(MDA) levels in cases and controls.Results(1)There were no difference in maternal clinical characteristics between the cases and controls.(2)Elevated maternal serum ROS level was found to be significantly much higher in pregnancies with IUH, regardless of singleton or twin pregnancies [singleton,(347.07 ± 125.28)IU/ml versus(312.41 ± 117.18)IU/ml, P<0.05; twin,(379.55 ± 169.12) IU/ml versus(311.76 ± 120.36)IU/ml, P<0.05]. And so was MDA [singleton,(5.86 ± 3.64) nmol/ml versus(4.46 ± 2.65)nmol/ml, P<0.05; twin,(7.42 ± 2.52)nmol/ml versus(4.58 ± 3.42) nmol/ml, P<0.05]. But no significant differences were found in SOD and GPx levels between the cases and controls.Conclusion IVF-pregnancies with intrauterine hematomas during the first trimester showed changes in oxidative stress level, which suggests a possible association with intrauterine hematoma and oxidative stress.