The Expression And Role Of Oxidative Stress Markers In The Serum，Follicular Fluid And Embryo Culture Media Of Obese Infertility Women

BackgroundAssisted reproductive technology（ART） has giving tens of thousands ofinfertile couples the chances of successfully nurturing children. However,obeseinfertility patients receiving ART is vulnerable to adverse pregnancy outcomes，in theform of low ovarian response, low implantation rate, low pregnancy rate and highabortion rate. The adverse pregnancy outcome of the molecular mechanism is still notclear. There is also no effective prevention and treatment measures. Therefore, toexplore the mechanism of the low success rate of obese infertile women receivingART can improve the pregnancy outcome of obese infertile women and the quality ofART.Oxidative stress (OS), widely found in the human body, is closely related tohuman aging and the occurrence of chronic non-communicable diseases. The body’stissues or cells generate active oxygen species (ROS) increasly and (or) oxidationresistance reduce, causing oxidative stress.There are two types of antioxidant systems:a class of antioxidant enzymes, and another class of non-enzyme antioxidants. Pastfew decades research results show that oxidation and antioxidant system,involved inphysiological and pathological processes of the body organs,are widely distributed inthe cardiovascular,respiratory,nervous,endocrine,immune, urinary, reproductive andother systems.Our previous work describes the expression of oxidative stress in serum and follicular fluid of patients with endometriosis and found that oxidative stress inserum and follicular fluid of those patients, suggesting that oxidative stress may playan important role in the reproductive system of patients with endometriosis. Thedevelopment of assisted reproductive technology has opened up new avenues for thestudy of eggs and embryos living environment. This study was to exploreserum,follicular fluid,embryo culture medium of obese patients in IVF-ET byenzyme-linked immunosorbent assay(ELISA),looking for potential clinicalapplications of drug and therapy.ObjectiveTo detect expression levels of oxidatie stress markers in serum, follicular fluid,embryonic medium of obesity infertility patients and try to find out the role of OS inobesity infertility patients.MethodsA prospective case-control study was conducted on94patients with tubal factorinfertility for IVF-ET.These patients were divided into the study group(47obesepatients) and the control group（47non-obese patients）.All patients in both groupsunderwent the mid-luteal long protocol ovulation. Peripheral venous blood andfollicular fluid were collected on the day of oocyte retrieval, embryo culture mediumwere collected on the day of embryo transferation.Reactive oxygenspecies(ROS),superoxide dismutase(SOD),and vitamin E(VE) concentrations infollicular fluid and serum were measured by enzyme-linked immunosorbent assay(ELISA). ROS in embryo culture medium were also measured. Compare the outcomeof IVF between the two groups: the number of oocytes retrieved, fertilization rate,good-embryo rate, implantation rate, clinical pregnancy, early abortion rate, combinedwith the level of oxidative stress markers were analyzed.Statistical methodsSPSS16.0software was used to analyze data,continuous data with normaldistribution are given as mean±SEM．One-way analysis of variance(ANOVA)on ranks was used to determine differences in ROS,SOD,VE contents．The differenceincidence rate of abnormal embryo was assessed by chi—square test．Line correlationanalysis between the experimental data and clinical evaluation was assessed byspearman correlation coefficient.Statistical significance was accepted at P<0.05．Results1. ELISA technology confirmed that the levels of ROS in serum of study groupwere significantly higher than the control group, SOD and VE levels in serum weresignificantly lower in study group(P＜0.05).ROS levels in follicular fluid wereslightly higher in the study group(P＞0.05)，whereas significnat differrence of SODand VE levels in follicular fluid was observed between groups(P＜0.05). Comparedwith control group, ROS in embryo culture medium of study group was slightlyhigher (P>0.05).In addition, ROS levels in serum of two groups were significantlyhigher in the embryo medium (P <0.05), the ROS level in follicle fluid of studygroup is higher than in embryonic medium (P <0.05);levels of SOD, VE in serum oftwo groups is significantly lower than in follicular fluid(P <0.05).2. There was no significant correlation between ROS，SOD and VE levels inserum,follicular fluid and embryo culture medium with IVF-ET outcomeevaluation(P>0.05).But ROS levels in serum and follicular fluid was a negativecorrelation with fertilization rate, positive correlation with the abortion rate; The levelof SOD and VE in serum and follicular fluid was positively correlated with thefertilization rate, implantation rate and clinical pregnancy rates trend,a negativecorrelation with the abortion rate.ConclusionsThe levels of ROS in serum,follicular fluid and embryo culture fluid wereelevated in the study group, and levels of antioxidant decreased, suggesting the obeseinfertility patients have oxidative stress exactly.