| Objective:Study on Clinical significance of local total immunoglobulin E(tIgE) and fungi specifi immunoglobulin E(sIgE) in sinusitis disease,and provide a theoretical basis for clinical diagnosis and treatment for sinusitis disease.Method:Study of 140 patients were treated in the First Affiliated Hospital of Anhui Medical University form November 2014 to October 2015, include of 30 cases of the control group(sinus tumor, nasal septum deviation, etc.), 60 cases of chronic rhinosinusitis(CRS) and 50 cases of non invasive fungal rhinosinusitis(NIFS). Chronic sinusitis were divided into 30 cases of chronic sinusitis without nasal polyps(CRSs NP) group and 30 cases of chronic sinusitis with nasal polyps(CRSw NP) group. Non invasive fungal sinusitis were divided into 30 cases of fungal ball(FB) group and 20 cases of allergic fungal sinusitis(AFRS) group. Using enzyme-linked immunosorbent assay(ELISA) to detect the expression level of tIgE in serum and tissue of all the research object. Using Immuno CAP100 system to detect the expression level of fungi(alternaria, white candida, specific penicillium, aspergillus, master more cigarettes spore, helminthosporium) sIgE in the serum and tissue of all the research object. SPSS19.0 statistical software was used for analysis, all the measurement data conforms to normal distribution with the mean and standard deviation(x±s),the two groups were compared by T test method. The qualitative data by chi square test, P<0.05 was statistically significant.Result:1. Serum tIgE(65.15 + 27.21KU/L) in CRSw NP, serum tIgE(70.66 + 27.09KU/L) in CRSs NP, were significantly higher than serum tIgE(56.56 + 5.32KU/L) in the control group, the difference was statistically significant(P<0.001).2. Tissue TIg E(124.30 + 4.40KU/L) in CRSs NP, tissue TIg E(235.10 + 4.21KU/L) in CRSw NP, were significantly higher than serum tIgE(56.56 + 5.32KU/L) in the control group, the difference was statistically significant(P<0.001). Tissue TIg E in CRSw NP was significantly higher than that of CRSs NP, the difference was statistically significant(P<0.001).3. The serum of fungal sIgE in CRSw NP was positive in 1 cases, detection of serum fungi sIgE in control and CRSs NP no positive results, calculation of fisherman accurate rate, P=1.0, the difference was not statistically significant. The tissue of fungal sIgE in CRSw NP was positive in 12 cases, compare with f 4 cases in CRSs NP and 3 cases in control group, by chi-square test respectively, c2=5.4 and c2=7.2, were statistically significant(P < 0.05).CRSw NP and the control group the number of positive cases by fisherman exact rate of algorithm P=1.0, the difference was not statistically significant.4. Serum tIgE(61.33 + 4.70KU/L) in FB, serum tIgE(260.36 + 5.95KU/L) in AFRS, were significantly higher than serum tIgE(31.97 + 21.13KU/L) in the control group, the difference was statistically significant(P<0.001).5. Tissue TIg E(122.11 + 4.24KU/L) in FB, tissue TIg E(485.75 + 9.69KU/L) in AFRS, were significantly higher than serum tIgE(56.56 + 5.32KU/L) in the control group, the difference was statistically significant(P<0.001). Tissue TIg E in AFRS was significantly higher than that of FB, the difference was statistically significant(P<0.001).6. The serum of fungal sIgE positive for 14 cases in AFRS, 1 case in FB and 0 case in the control group. AFRS compared with FB and control group in serum fungal sIgE positive cases respectively, by chi-square test, c2=25.4 and c2=29.2,were statistically significant(P<0.001). FB and the control group the number of positive cases by fisherman exact rate of algorithm P=1.0, the difference was not statistically significant. The tissue of fungal sIgE positive for 20 cases in AFRS, 4 case in FB and 3 case in the control group. AFRS compared with FB and control group in tissue fungal sIgE positive cases respectively, by chi-square test, c2=33.3 and c2=36.1,were statistically significant(P<0.001). FB and the control group the number of positive cases by fisherman exact rate of algorithm P=1.0, the difference was not statistically significant.Conclusion1.The tissue IgE is involved in sinusitis disease, particularly related to the formation of nasal polyps and AFRS. 2. CRSw NP and AFRS associated with local fungal allergy, provide the basis for clinical fungus desensitization treatment. 3. Local tissue tIgE and fungal sIgE detection more sensitive than serum in sinusitis disease, and provides a new method for clinical diagnosis of allergic diseases. |
