The Role Of Superior Olivary Complex Related Proteins In Central Auditory Processing

Objective:The proteomic analysis of 2-D DIGE and iTRAQ technique were used to analysis the differential proteins of superior olivary complex (SOC) in SD rats.Methods:The cochlear nuclear complex (CN), SOC and inferior colliculus (IC) were isolated from P60 healthy male SD rats, the rest of brain was used as control. The 2-D DIGE and iTRAQ technique were respectively used to examine and identify the differential cytoplasmic fractions and plasma membrane-enrich fraction of SOC, the function of these proteins were analyzed by bioinformatics, such as Gene Otology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).Results:26 cytoplasmic protein spots in SOC were found by 2-D DIGE image comparison analysis. Then 17 cytoplasmic proteins of SOC were detected by mass spectrometry. A total of 1937 brainstem membrane proteins were identified by iTRAQ technique, and 14 different membrane proteins were confirmed in SOC after comparative analysis. Bioinformatics analysis of cytoplasm and plasma membrane differentially expressed proteins in SOC revealed that, Alanine--tRNA ligase, glutamyl prolyl Trna synthetase, Alpha-internexin and Choline transporter-like protein 1, Tubulin alpha-4A chain mainly involved in the development of nervous system, nerve transmission and synaptic plasticity, and the rest of these proteins were mostly related to energy metabolism.Conclusion:In SOC, Alanine--tRNA ligase, glutamyl prolyl Trna synthetase, Alpha-internexin, Choline transporter-like protein 1 and Tubulin alpha-4A chain proteins may be related to the central auditory processing. They provided theoretical basis for further research on the pathogenesis of central auditory processing disorder.