| Objective: To investigate the effect of calcium gluconate, morphine, antondine pre-emptive injection on the behaviors and expression of pronociceptive mediators of rats poisoned by latrodectus tredecimguttatus spiders’ toxin. Methods: 120 male SD rats were divided into three groups randomly:calcium group, morphine group and antondine group, which were injected each of analgesic drug before poisoned by different concentration of latrodectus tredecimguttatus spiders’ toxin. The nocice ptive response and flinching times were observed and recorded. The expression of pronociceptive mediators, including P2X3 receptor, SP, CGRP, in spinal cord were detected by immunohistochemistry. Results:(1) When the toxin concentration is 10μl/kg, the analgesic effect of morphine is stronger than that of antondine, while the calcium group is the worst at 15 min. And at 30 min the effect of morphine and antondine are superior to the effect of themselves at 15 min, while the effect of calcium is the best. When the concentration is 300μl/kg, the analgesic effect of calcium is much stronger than that of morphine and antondine(P<0.05);(2) The expression of P2X3 receptor of calcium group was lower than that of morphine and antondine group prominently. The expression of SP in all of three groups is feminine in the spinal cord; The inhibition of CGRP of calcium and antondine are superior to morphine. Conclusions: The analgesic effect of calcium, morphine and antondine have their advantages at different concentration and time, the inhib-ition of P2X3 receptor and CGRP of calcium to relieve pain is more powerful th-an morphine and antondine. All of three kinds of analgesic drugs could eliminate the expression of SP. |
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