| Background and Objective:Tonsils located at the intersection of digestive and respiratory tracts. There are a lot of lymphoid tissue in Mucosal here. It often contact with the antigen and cause local immune response. Tonsillar hypertrophy pathological hypertrophy because repeated stimulation tonsil tissue of inflammation. Chronic tonsillitis is one of the most common diseases. Because recurrent tonsillitis or tonsil crypts poor drainage. There are a lot of bacteria, viruses causing infection in the nest. Tonsil cancer is a common malignant of oropharyngeal. In the upper respiratory tract malignant tumors it after the incidence of throat, The cause is not clear, may be related with long-term inflammatory stimuli, pre keratosis, vitiligo and other cancer diseases and smoking, drinking and other factors Mostly occurs in men. Male to female ratio is 2-3:1. With the development of virology and biology, it was discovered that the incidence of cancer are closely related with viral infections. EB virus is also confirmed that it is associated with neoplastic diseases. The study also found that EBV is closely associated with a variety of tumors of epithelial origin. In terms of tonsil cancer, there are less related reports about human papilloma virus (HPV), cytomegalovirus (CMV), EB virus (EBV). The purpose of this study is to detect the existence of the human papilloma virus (HPV) and chronic tonsillitis organizations, cytomegalovirus (CMV), EB virus (EBV) in tonsil cancer, tonsil hypertrophy and chronic tonsillitis organization. Discussion the pathogenesis of EB virus (EBV), cytomegalovirus (CMV) and human papilloma virus (HPV) in tonsillar hypertrophy, chronic tonsillitis and tonsil cancer disease.Methods:In this study, the specimens are specimens of tonsils, chronic tonsillitis and tonsil cancer which are surgical removaled in the Department of Otolaryngology Head and Neck Surgery in first People’s Hospital of Zhejiang Province in June 1,2014-March 31, 2015. A total of 46 cases, including 29 cases of tonsillar hypertrophy specimens,11 cases of chronic tonsillitis specimens and six cases of tonsil cancer specimens. The samples after removing are stored frozen in liquid nitrogen. Using fluorescence quantitative PCR technology to detect EBV-DNA, CMV-DNA, HPV (16,18,31,33, 35,39,45,51,52,56,59,68 type)-DNA in 29 cases of tonsillar hypertrophy,11 cases of chronic tonsillitis and six cases of tonsil cancer tissue. Using method of Fisher’s exact test and X2 test study results and statistically analyzed. P<0.05 was considered standard of statistically significant. Results:Using fluorescence quantitative PCR technology to detect the positive rate of EBV, CMV, HPV (16,18,31,33,35,39,45,51,52,56,59,68 type) in tissue specimen. In tonsil cancer, the positive rate was 100%(6/6), respectively,16.7%(1/6) 0%(0/6). In chronic tonsillitis, the positive rate was 81.8%(9/11) and 0%(0/11) and 0%(0/11).In tonsils, the positive rate was 79.3%(23/29),13.4%(1/29),0%(0/29). Compare with chronic tonsillitis, EBV infection rate (P<0.05) higher in the tonsils carcinoma, and CMV, HPV was no significant difference (P> 0.05). Compare with tonsillar hypertrophy tissue, EBV infection rate (P<0.05) higher in the tonsils carcinoma, and CMV, HPV was no significant difference (P> 0.05).Conclusion:1. The positive rate of EBV expression in tonsillar carcinoma is higher than in chronic tonsillitis organizations or in tonsillar hypertrophy tissue. Tip EBV infection may be closer with tonsil cancer than chronic tonsillitis or tonsillar hypertrophy.2. The expression of CMV, HPV are no significant difference in tonsillar cancer, chronic tonsillitis and the tonsillar hypertrophy. Tip the infection of CMV, HPV was no correlation with tonsil cancer, chronic tonsillitis, tonsillar hypertrophy. |
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