Study Of Association Between STEAP4 Gene Polymorphism And Metabolic Syndrome

ObjectiveAltered expression of STEAP4 is linked to obesity, insulin insensitivity, metabolic homeostasis, and inflammation. This study assessed STEAP4 SNPs for association with a risk in developing metabolic syndrome in Han Chinese population. MethodsA total of 3375 Han Chinese subjects were included in this case-control study with 1583 metabolic syndrome(Met S) and 1792 healthy controls. Four SNPs(rs1981529, rs2040657, rs10263111, rs12386756) were genotyped using PCR and MALDI-TOF-MS. The association between STEAP4 SNPs and Met S was then analyzed statistically by SPSS 19.0 and SNPStats. Results1.In this study, we recruited a total of 3375 Han Chinese individuals, among these subjects, there were 1583 cases(799 males, 50.5%, 784 females, 49.5%, male/female ratio 1.02:1) and 1792 controls(899 males, 50.2%, 893 females, 49.8%, male/female ratio 1.01:1). In the case and control groups, there was no significant difference in the age(χ2=0.032,P=0.863) or gender(t=-0.385,P=0.700).2.We found that all SNPs of this gene were in accordance with Hardy-Weinberg equilibrium(P>0.05), except rs12386756, whose genotype frequency did not conform to the HWD test(P<0.05). There was no statistical difference in allele frequency of these four SNPs between the case and control(P>0.05). The genotype of rs12386756 was shown to be significantly associated with Met S(χ2=6.698,P=0.035). Other three SNPs(rs1981529, rs2040657, and rs10263111) did not show any statistical difference in genotype distribution in both the case and control groups(P>0.05). Compared with the AA / GG genotypes, the GA genotype of rs12386756 significantly increased the risk of developing Met S(OR=1.297, 95% CI 1.065-1.580, P=0.010).3.There was also no haplotype that could be associated with the risk of developing Met S(P>0.05).4.Furthermore, SNP rs1981529 of STEAP4 was associated with BMI(CC<CT<TT, F=3.886, P=0.021), waist circumference(CC<CT<TT, F=3.728, P=0.024), and SBP(CT<TT<CC, F=3.168, P=0.042), while SNP rs10263111 was associated with waist circumference(GG<CG<CC, F=3.270, P=0.038) and fasting glucose(GG<CG<CC, F=3.769, P=0.023) levels. SNP rs12386756 was associated with waist circumferences(GG<GA<AA, F=3.739, P=0.024) and hip circumferences(GG<GA<AA, F=4.253, P=0.014).5.There was no statistical difference in interaction between four SNPs and environmental factors(P>0.05). Conclusion1.Based on our data, we inferred that STEAP4 rs12386756 has a higher risk of developing metabolic syndrome in this Han Chinese population.2.We found that SNP rs1981529 of STEAP4 gene was associated with BMI, waist circumference, and SBP, while SNP rs10263111 was associated with waist circumference and fasting glucose levels of subjects with Met S.3.There was no statistical difference in interaction between four SNPs and environmental factors. Further studies must be conducted to understand the role of STEAP4 gene in the pathogenesis of metabolic syndrome.