Study Immune Regulation Mechanism "Fei Chang He Zhi" Intestinal Flora Merger Allergic Asthma Rat Airway Remodeling

Objective:This subject establish rat intestinal flora imbalance merger on the basis of allergic asthma, lung Chang He Zhi to representatives of prescription flavored Shengjiangsan intervention, through the observation of this animal experiments, selection and airway weight plastic related to a number of indicators, to explore combined treatment method for lung intestinal intestinal flora merger allergic asthma rat airway remodeling, the "lung and Large Intestine" organs theory to find empirical basis.Methods:In SD rats for the study, antibiotic-induced intestinal flora model using ovalbumin (OVA) based on aluminum hydroxide (Al (OH) 3) suspension in rats with bilateral chest, groin and intraperitoneal injection and inhalation stimulate asthma, intestinal flora merger establish rat model of allergic asthma. Modified Shengjiangsan after the intervention, through the lungs, intestines synchronous observation research ideas, with pathology observed intestinal pathological changes of lung tissue were detected by ELISA in serum and bronchoalveolar lavage fluid in rats (BALF) TGF-β1, IL-17 content; immunohistochemical staining of lung, intestine MMP-9, TIMP-1 content ratio.Results:1. ethology:the intestinal flora merger allergic asthma model group rats compared to rats difficulty breathing medicine Fei Chang He Zhi intervention group, runny nose, scratching and other symptoms have abated different; in the breeding process, Rats coat gloss, the overall situation is good;2. Pathologic shows lungs of rats intestinal combined group bronchial wall and surrounding tissue inflammatory cell infiltration, epithelial damage than the model group reduced intestinal tissue damage in each group inflammation and intestinal mucosa are more models group remission;3. The lung function test in the model group breathing frequency (F) compared with the control group was significantly higher (P<0.05)), minute ventilation (MVb), peak inspiratory flow (PIFb) peak expiratory flow (PEFb) was significantly reduced (P<0.05 or P<0.01). Compared with the model group, lung intestines of rats can reduce the combined group F (P<0.05 or P<0.01), increased MVb, PIFb, PEFb (P<0.05 or P<0.01);The model group rats and intestinal mucus in BALF sIgA were significantly lower (P<0.05 or P<0.01) the control group; compared with the model group, positive group and BALF sIgA content and intestinal mucus in the treatment group than the model group liter higher (P<0.05 or P <0.01);5. Cytokine levels1) serum and BALF in model group TGF-β1, IL-17 was significantly higher than the control group largeMice (P<0.05 or P<0.01); compared with the model group, positive group of intestinal and lung combined group of TGF-β1, IL-17 have different degrees downward effect (P<0.05), with a statistically significant difference significance.2) Immunohistochemistry results show:the model group and the blank group MMP-9/TIMP-1 ratio imbalance severe (<1); positive group increased intestinal and lung combined treatment in the intervention group compared with model group MMP-9 levels is slow, TIMP-1 levels increased significantly (P<0.05); of MMP-9/TIMP-1 ratio close to normal, there is a significant regulatory role.Conclusion:Improve lung Chang He Zhi intestinal flora merger allergic asthma rat airway remodeling adjustment mechanism may have two:First, by reducing lung infiltration of inflammatory cells, to repair the airway mucosal injury, and then restore damaged tissue structure; Second, regulation of TGF-β1, IL-17 expression, reduce lung tissue MMP-9, TIMP-1 levels, regulate MMP-9/TIMP-1 ratio tends to balance up early antagonize airway remodeling.