| Objective:This research based on "lung and e phase exterior" related viscera theory, established "Lung intestinal collection disease" model that intestinal bacteria group disorders merged allergy sex asthma animal model, combines Th17/Treg balance mechanism in asthma occurred development in the vital of role, compared rule lung, and rule intestinal, and lung intestinal collection rule three species rule method on intestinal bacteria group disorders merged allergy sex asthma of treatment effect, and and on Thl7/Treg balance mechanism regulation role, this will further rich "lung and e phase exterior" viscera related theory of science connotation, Also provides clinical TCM for prevention and treatment of allergic asthma with theories and ideas of reference, with a view to improve clinical outcomes.Methods:Selection clean level Sprague-Dawley male big rat, by weight for layered design random group, is divided into blank group, and bacteria group disorders merged asthma inspired Group (Hou referred to model group), and "lifting bulk" treatment group (Hou referred to lung intestinal collection rule group), "Guizhi plus Magnolia apricots soup" treatment group (Hou referred to rule lung group), "increased liquid bearing gas soup" treatment group (Hou referred to rule intestinal group), total five group. According to the reference established after the modified method of intestinal dysbacteriosis with allergic asthma model. After the model, students from the three treatment groups were given the same day lift powder, apricot cinnamon twig decoction adding Magnolia bark decoction, zengye Chengqi decoction drug 10ml intragastric administration of concentrated liquid, empty groups, and model set irrigation with saline for 7 consecutive days. General conditions of experiment rat (in appearance, reaction, color, na food, faeces, and so on), asthma symptoms (irritation, sneezing, coughing, abdominal muscles strong contractions, breathing, etc); rats blood, urine and stool routine, blood biochemical indexes and so on. Lung tissue and colonic tissue, bronchial alveolar lavage fluid, fresh feces in the colon. Immunohistochemical observation of lung tissue inflammation, BALF QPCR detection GATA-3 mRNA,T-betmRNA,Foxp3 mRNA,ROR-mRNA,TGF-βγ expression of tmRNA, ELLIS A detection in BALF of IFN-y cytokines, IL-6, IL-17, IL-4, concentration and sIgA content of bronchial mucosa and intestinal mucosa.Result:1. Big rat general situation changes:model group, and rule lung group, and rule intestinal group, and lung intestinal collection rule group of big rat in atomization process in the are appeared different degree of upset upset or quiet less moving, and breathing frequency accelerated deepened, and nodded sample breathing, and buck erect, and forelimb shrink lift, II will incontinence, asthma speed sent instead should performance; normal group big rat is activities freely, no above performance; except blank group outside, model group, and rule lung group, and rule intestinal group, and lung intestinal collection rule group are different degree appeared lazy moving, and less food, Burnout curled up, was hair fluffy no Ze, reaction slow, Weight loss, reduce the amount of food intake, stool frequency increases or decreases, then quality thin loose stools, occasional constipation and bowel problems.2. Morphology of lung tissue and intestine tissue results:compared with the blank group and lung tissue of model groups around the blood vessel wall and Lung between mass inflammatory sex cell infiltration serious, after treatment Hou, disease loss situation has different degree of change, which to rule intestinal group of lung organization improved most significantly, second for lung intestinal collection rule group; and model group of colon organization more blank group muscle layer, and serous layer inflammatory cell infiltration are different degree damage, treatment 3 Group on colon organization disease loss has improved, which rule lung group than pathology model group infiltration degree reduce most significantly, second is for lung intestinal collection rule group.3. Inflammation of the bronchi of the lungs changes:BALF EOS percentage significantly lower in the treatment of pulmonary group, white blood cell counts and EOS also has decreased, but no significant difference; IL-4 in BALF was reduced significantly. EOS or EOS in BALF in treating bowel group percentage decrease, with significant differences; IL-4 in BALF was reduced significantly. Lung and intestinal EOS in the BALF were significantly lower in the treatment group, white blood cell counts and percentage EOS also has decreased, but no significant difference; IL-4 in BALF was reduced significantly.4. Changes of pulmonary function:treatment of pulmonary group breathing in asthmatic rats was significantly lower than the model group, closer to the group level was statistically significant. Although airway hyperresponsiveness model group, but not a statistically significant difference. Governance Group of intestinal respiration in rats with asthma and airway hyperresponsiveness were significantly lower than the model group, statistically significant. And treating group of asthmatic rat lung and intestinal respiration and airway hyperresponsiveness were significantly lower than the model group, statistically significant.5. Changes in intestinal flora:the amount of coliform bacteria in the treatment group compared with the model was significantly reduced, making it more likely to blank level, number of Lactobacilli and Bifidobacteria are significantly increased, significantly reduced Candida albicans, are statistically different. Compared with the model of Enterococcus group, but not statistically significant. Treatment of intestinal Lactobacilli and Bifidobacteria in the group numbers were significantly increased, significantly reduced Candida albicans, are statistically different. Enterococcus faecalis and Escherichia coli Group changed little, not statistically significant. Lung and intestinal Escherichia coli in treated groups compared with the model number of the Group decreased number of Lactobacilli and Bifidobacteria are significantly increased, significantly reduced Candida albicans, are statistically different. Compared with the model of Enterococcus group are declining, but not statistically significant.6.Changes of intestinal sIgA:sIgA treatment group compared with the model groups increases, but without statistical significance. Treatment of intestinal sIgA were significantly increased compared with the model group, there was significant. Lung and intestinal sIgA treatment group than in the model group increased significantly, with statistical significance.7 Serum in the IL-4, and IL-6, and IL-10, and IL-17 content of changes:rule lung, and rule intestinal, and lung intestinal collection rule group are significantly increased has serum in the IL-10 of level, to rule intestinal group most obviously; rule lung, and lung intestinal collection rule group are reduced has serum in the IL-17 of level, to rule lung group more obviously, rule intestinal group although performance out on serum in the IL-17 of level has reduced trend, but no statistics meaning; rule lung, and rule intestinal, and lung intestinal collection rule group are reduced has serum in the IL-6 of level, To treat intestinal group is obvious. Treating lung, intestine, lungs, intestinal treatment of treatment groups are to varying degrees in elevated serum levels of TGF-β to curing lung group most notably, lung and bowel treatment increased the most inconspicuous.8. PCR assay for detection of lung tissue expression of FoxP3mRNA, ROR y tmRNA change:treating lung, intestine, lungs, intestinal treatment of treatment groups showed elevated levels of FoxP3mRNA and ROR gamma level of tmRNA reduced FoxP3mRNA elevated levels of lung and bowel treatment groups is the most obvious, ROR y tmRNA levels lower in the treatment group is most obvious.Conclusion:1. Treating lung, intestine, lungs, intestinal treatment of treatment of three therapy of intestinal dysbacteriosis with allergic asthma rats showed treatment. Method of treating lung on behalf of parties "apricot cinnamon twig decoction adding Magnolia bark decoction" can significantly improve the model changes of intestinal microflora in rats and may increase levels of intestinal slgA. Method of treating intestinal representative "zengye Chengqi decoction" can significantly improve lung inflammation and pulmonary function. Lung and bowel treatment method can also improve the function of pulmonary inflammation, lung, intestinal flora and intestinal slgA level changes.2. Treating lung, intestines, lungs of treatment treating three kinds of therapeutic methods on intestinal gut flora Th17/Treg balancing mechanisms in rats with allergic asthma related Cytokines and genes have shown positive effects. Method of treating bowel may have elevated serum levels of IL-10, lower serum IL-6 levels and plays a benign regulatory Thl7/Treg the balance mechanism is effects; treating lung law may reduce serum levels of IL-17, elevated serum levels of TGF-β and reduces lung ROR y tmRNA mechanism plays a sound level Th17/Treg balance effect Lung lung tissue in intestinal treatment group may be increased by regulation of expression and play a benign Th17/Treg FoxP3mRNA level balancing mechanism is effects.3. This research has further enriched "the lung and the large intestine are internally" the scientific connotation of Zang-Fu theory, demonstrates that the lungs and intestines in physiological and pathological conditions and degeneration. In clinical treatment intestinal bacteria group disorders merged allergy sex asthma Shi, on lung inflammation and lung function poor more significantly who should be selection or plus with rule intestinal method; on intestinal bacteria group disorders more serious who should be selection or plus with rule lung method treatment; on while appeared lung inflammation, and lung function poor, and intestinal bacteria group disorders and intestinal immunity reduced more significantly who should be selection lung intestinal collection rule method treatment. |
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