Study On The Constituents And Bioactivity Of Compound Black Ginseng

The objective of this experiment determines the compound process of black ginseng,studys and evaluates quality and activities of black ginseng. The results are as follows:1.Make preparations of Black ginseng.Put the dried ginseng into garlic juice for 12 h,and let it in the high temperature and high pressure circumstance for 2 h.Drying under reduced pressure oven with 40 for 12 h to kill sterilization and dry ℃by stream and then obtain the compound of heishen.2.Determination of rare ginsenoside.From the HPLC map we can know that about 8-30 min appeared rare ginsenoside Rh1,Rg3,PPT,Rh2,PPD,and the other 5 kinds of rare ginsenoside which with much content.They are respectively 0.29 %、0.34 %、0.14 %、0.96 %、0.62 %.HPLC compound black ginseng spectrum many unknown peaks also appear in the graph. The peak concentrations are very high,and more intensive. They are may be the product of ginseng after hydrolysis. They have value to research and can be further studied.3.The kinds and content of amino acids determination.The content of compound black ginseng amino acid are varied and comprehensive which with 21 kinds of amino acids in the determination of amino acids. compound black ginseng except containing glutamic acid and higher levels of threonine and proline and alanine. It contents 2548.21 mg/100 g;arginine contents 936.13mg/100 g.4.Determination of total sugar and reducing sugar. Compare the content of compound black ginseng to the total sugar in ginseng. The content of reducing sugar increased significantly,compound black ginseng reducing sugar content as high as 61.07 %,while only 3.13 % of ginseng,total sugar and reducing sugar of 92% Compound black ginseng and ginseng,reducing sugar content rarely,only account for 6.4% of the total sugar.5.Determination of DPPH Radical Scavenging Activity and the total phenolic content.The total phenolic content in compound black ginseng higher than total phenolic content of 100 g.Compound Black ginseng can exact to 1.02 g. Extracting the compound black ginseng can effectively scavenge DPPH free radical,and showed the obvious effect relationship. The scavenging rate was 93.04 %. It is that Compound black ginseng extract has stronger antioxidant ability.6.Determination of α-glucosidase inhibitory activity.Compound black ginseng extract m certain α-Glucosidase Inhibitory Activity of IC50 value is 1.09 mg·mL-1.7.Determination of ACE inhibitory activity and nitrite scavenging effect.Compound black ginseng extracts only 50% ethanol extracts of 30% ethanol extracts methanol and extracts some inhibitory activity of ACE. inhibitory activity,half of which are as follows:26 mg·mL-1, 5 mg·mL-1, 7 mg·mL-1; methanol and water two abstraction layer and 30% ethanol, 50% ethanol ultrasonic extract of black ginseng on nitrite showed certain ability of scavenging,IC50 values were 0.606,0.439,0.107,0.303 mg,mL-1 results can be summarized by the scavenging effect of compound black ginseng with nitrite of certain.8.Acute toxicity test.Compound black ginseng mice acute toxicity experiment results show that the toxicity of compound black ginseng is extremely low,did not detect LD50;compound black ginseng according to the maximum concentration shows the maximum dose administration,mice do not appear dead and obvious poisoning,so the compound black ginseng with high security.9.Anti fatigue test.Compound black ginseng had no obvious effect on mice body mass and can prolong the swimming time of mice,increase the content of liver glycogen,muscle glycogen in mice,decrease the concentration of serum urea nitrogen,increased serum lactate dehydrogenase activity,reduce the content of lactic acid,has significant anti fatigue effect.