CWP Rabbit With High-resolution CT Manifestations And Serum TGF β 1 Expression Level Related Research

Objective:Hangs the fluid manufa CTure coal labor pneumoconiosis with certain quality coal dust the animal model, observes its course of an illness through phantom study method(high-resolution CT) to progress, simultaneously examines the animal blood serum transformation growth fa CTor β1(TGF-β1), and displays to the blood serum TGF-β1 expression and high-resolution CT in the coal labor pneumoconiosis course of an illness progress aspe CT relevance condu CTs the research, inquires into to high-resolution CT at the coal labor pneumoconiosis diagnosis value and with TGF-β1 the expression relevance.Methods:1, Sele CT weight for rabbits 40 2-3kg the same property, fully 25 randomly sele CTed only as a group, while the remaining 15 only as a control group, and then fully in the experimental group and the control group were randomly divided into groups of five, the obje CT corresponding to each issue as a CT scan. Animals in the experimental group in the neonatal endotracheal intubation laryngoscope-guided100 ml dust <5μm in diameter in the suspension of coal dust into the lungs in the control group in the same method, the dose of saline into the lungs.2, To makes in front of the mold all rabbits and make the mold latter 2 week, 1month, 2 month, 3 month, 5month corresponds five groups of rabbits in theexperimental group and the control group internal phase to carry on the CT scanning,and carries on the image reconstru CTion analysis with the high resolution bone reconstru CTion algorithm, the parameter is: Level thick 2mm, spacing 0.6mm, the window extends 1350 Hu, window position 500 Hu, and has the demonstration following chara CTeristic to the lung window in to carry on the recording: Bronchial tube blood control exceptionally, ground glass shade, septum interlobulare accumulation, honeycomb shade, widespread ring-like phantom.3, Each of the scanning of rabbit rabbit ear after the harvest blood, rabbit serum was obtained by centrifugation after standing by the double antibody sandwich ABC-ELISA method using rabbit transforming growth fa CTor β1(TGF-β1) ELISA kit TGF-β1 content.Results:Experiment Group and the high-resolution CT performance are as follows: 2weeks, 1 Lung, the ground glass, density, and bronchial blood Control Exception,botanists interval thickening, Lung-quality fiber, the image chara CTeristics of performance; 1 months to 3 months, to be observed in the lung-quality fibers, image features are more up-front; 3 month to 5 month period of cases cellular shade and broad ring; control group is not clear. By double-antibody sandwich ABC- ELISA the Measurement Experiment group and control group for the serum TGF β1 content,the more experimental and control groups each time TGF β1 content, the analysis of analysis of variance analysis: inter-group F = 65.803, P <0.001, which can be considered experimental group TGF β1 Content comparison group; group time intera CTion analysis F = 2.782, P = 0.045, which can be considered experimental group TGF-β1 content with the passage of time increases. TGF β 1(PG/ML) and high-resolution CT serious degree of correlation coefficient RS = 0.908, P = 0.033,which is considered to be TGF β 1 and high-resolution CT severity is related.Conclusion:Each of imaging changes rabbit coal pneumoconiosis by high-resolution CT observed and correlated with serum TGF-β1 content of coal pneumoconiosis model rabbit serum TGF-β1 with the progression of gradually increasing trend.