Association Between NPR3 Gene Polymorphisms And Essential Hypertension In Chinese Han Population

Objective To research the association between natriuretic peptide receptor-C(NPR3) gene polymorphisms and essential hypertension(EH) in Chinese Han population.Methods We designed a case-control study. This study population consisted of 452 EH patients and 434 normotensive controls with the similar age and rate of sex. The history of smoking, drinking, type2 diabetes mellitus, and familiar history of EH were questioned. All participants were checked by blood biochemistry analysis, including total cholesterol, triglyceride, low-density lipoprotein cholesterol(LDL-c), high-density lipoprotein cholesterol(HDL-c), fasting blood sugar(FBS), uric acid(UA), blood urea nitrogen(BUN), creatinine, Na+, and Ka+. Nine SNPs were captured by the way of Tag single nucleotide polymorphism(TagSNP),including rs3792758, rs1173773, rs13436831, rs11750438,rs1173747,s2292026,rs976576,rs696831,and rs3811953.The technique of polymerase chain reaction( PCR) and multiple SNaPshot PCR were used for gene amplification.Genotypes were checked by means of ABI 3130 XL gene analyzer, and the peakmap were drawn by the software of Peak Scanner 1.1. Statistical analysis were used by Student t test, chi-square test, and logistic regression analysis. Moreover, two-sided values of p <0.05 and 95% confidence interval were used to test the statistical significance of between-group differences, performing by the SPSS 18.0 statistical program. The Hardy-Weinberg(HW) analysis was detected by X2 test and Linkage Disequilibrium(LD) was analyzed by the java of Haploview 4.2.Results The genotypes at nine loci were in agreement with Hardy-Weinberg expectation in both cases and controls. Rs1173773 GG carriers had a 3.164-fold increased risk of EH than nonGG carriers(P =0.037) by binary logistic analysis. After adjusted by age, sex, smoking, drinking, waist circumference, Body Mass Index(BMI) and familiar history of EH(OR = 3.012,95% CI = 1.002-9.728, P =0.041),the association was still positive(P<0.05). However,the remainder of SNPs were all not significant(P>0.05). Furthermore, all nine SNPs allelic genes frequent distributed between cases and controls were no significance. In the subgroup analysis, Rs1173773 GG carriers with EH had a 3.509-fold increased risk of high BMI than non-GG carriers(P =0.049). The subgroup statistical analysis was no significance difference between G or A allelic gene. Additionally, whether EH accompanied with DM or not, there was also not significant difference between G or A allelic gene.Conclusions In Chinese Han population,NPR3 gene rs1173773 GG carriers may increase the risk of hypertension, and have a possible influence of higher BMI than non-GG carriers in cases.