The Relation Between Plasma Kallikrein B1 And Bradykinin B2 Receptor Gene Snp, Bmi And Essential Hypertension

Objective: Essential hypertension (EH) is a complicated disease which is affected by multiple factors. It is an important cause and risk factor in many kinds of cardiovascular and cerebrovascular diseases. The onset of EH is notably impacted by the inherited factor. Plasma kallikrein B1 (KLKB1) and bradykinin receptor B2 (BDKRB2) play an important roles in regulating blood pressure and renal sodium. KLKB1 and BDKRB2 genes are candidate genes which contribute to EH morbidity. Recently, it was reported that the relationship between EH and single nucleotide polymorphism (SNP) of KLKB1 and BDKRB2 genes was in many case-control study literatures. But it was inconsistency in different races and regions, furthermore it was seldom reported in geratic Han people of essential hypertension. In addition, epidemiologic studies were showed that the onset of EH was obviously related to body mass index (BMI). However, it was not reported to the synergistic action between BMI, KLKB1 and BDKRB2 gene polymorphism. Our questions for study and discussion are the correlation between EH and two SNPs (rs1799722 and rs2304595), and the reliable evidence of the association of EH onset risk and two SNPs in order to provide us the effective methods of prevention and cure for EH, especially geratic EH.Methods:1. Restriction fragment lenth polymorphism and polymerase chain reaction (RFLP-PCR) were used to detect KLKB1 gene SNP(rs2304595 A/G) in 103 geratic normotension controls and 103 geratic EH patients at Chongqing region. Statistical analysis was performed to investigate the correlation between KLKB1 gene SNP, BMI and geratic EH.2. Restriction fragment lenth polymorphism and polymerase chain reaction (RFLP-PCR) were used to detect BDKRB2 gene SNP (rs1799722) in 103 geratic normotension controls and 103 geratic EH patients at Chongqing region. Statistical analysis was performed to investigate the correlation between BDKRB2 gene SNP, BMI and geratic EH.3. In electronic databases , the literatures of case-control trials of the relation between BDKRB2 gene SNP(rs1799722T/C) and the EH risk were searched, which met the inclusion criteria.Statistical analysis was performed with RevMan 5.0 and SPSS 13.0 to investigate heterogeneity, to use suitable models for the study data combination and effect evaluation and to undertake the analysis of ethnic subgroups and the evaluation of publication bias.Results:1. The odds ratio(OR) for GG genotypic carriers of KLKB1 gene SNP(rs2304595) was 1.60, furthermore geratic Han people cases and contrals showed no statistical difference(P>0.05) at Chongqing region. The male subgroups of GG genotypic carriers were no statistical difference(P>0.05); but the female subgroups of GG genotypic carriers were significant statistical difference (P<0.01). Theχ2 test showed that the EH risk of geratic individuals of BMI≥25 were significant statistical difference(P<0.01). Logistic regression analysis indicated that rs2304595 GG genotype and BMI had certain synergistic action to increase the onset risk of geratic EH at different levels, and the onset risk influenced by GG genotype became even bigger in Chongqing local geratic Han people.2. The OR for CC genotypic carriers of BDKRB2 gene SNP (rs1799722) was 0.74 at Chongqing region. The male or female subgroups as well as all groups of CC genotypic carriers were no statistical difference(P>0.05), and CC genotype didn't precisely affect the geratic EH risk in Chongqing local geratic Han people.3. Totally, 10 literatures, 12 studies, 1736 cases and 1682 controls were included. The Meta analysis showed that the EH risk of CC genotypic carriers of BDKRB2 gene SNP(rs1799722) was 1.36 times than CT or TT genotypic carriers(P<0.05). Japanese, Chinese Han and African-American CC genotype were related to the EH risk(P<0.05); but the association between the EH risk and CC genotype of Chinese Kazakans, partial Japanese and Caucasian was indefinite in the subgroups analysis(P>0.05).Conclusions: The onset risk of geratic EH of KLKB1 gene rs2304595 GG genotypic and allelic carriers was increased notably at Chongqing region, furthermore GG genotype and BMI had synergistic action in the geratic EH morbility. The association between CC genotype of BDKRB2 gene rs1799722 and Chongqing local geratic EH morbility was indefinite, furthermore the Meta analysis showed that the CC genotype of BDKRB2 gene rs1799722 might increase the EH risk, but this conclusion was notably affected by local and ethnic differences.