Studies On The Chemical Constituents Of Dysosma Versipellis

Dysosma versipellis, belonging to Dysosma genus, Berberidaceae, was selected for phytochemistry and pharmacologyical investigations. D. versipellis was distributed in Guangxi, Jiangxi, Hubei, Sichuan and Guizhou province.The plants of Dysosma genus are widely used as a Chinese traditional medicine, which has high medicinal value. Modern pharmacological experiments show that D. versipellis has a wide range of physiological activities, such as antitumor, antiviral, antibacterial and anti venom. Podophyllotoxin, one of the most important chemical constituent of the genus, also shows significant pharmacological activity.Stems and roots of D. versipellis were isolated and purified in a combination of chromatographic methods(silica gel column chromatography, Sephadex LH-20 gel column chromatography, and preparative HPLC, etc). The isolated compounds were identified by spectroscopic data analysis(ESI-MS, 1D and 2D 1H NMR and 13 C NMR, IR, and CD spectra). Totally 40 compounds were separated from D. versipellis, including 24 lignans, 9 flavonoids and 7 others. Among these compounds, there are 9 new compounds, including two new norlignans(DV-1 and DV-2), a new lignan(DV-20), six new flavonoids(DV-25? DV-30)。At the same time, 12 compounds were isolated from the genus the first time.The antibacterial properties against Riemerella anatipestifer, Bacillus subtilis, Enterococcus faecalis, Pseudomonas aeruginosa, Aeromonas hydrophila, Streptococcus agalactiae, Streptococcus suis of six new flavonoids along with three known compounds was assessed. They all show different antibacterial activities, and it was deduced that the cyclization of the isopentene group will increase the antibacterial activity.