Study On Alcohol Extraction Condition And Effects On Nerve Cells Of Kaixinsan

Kaixinsan contains ginseng, rhizoma acori graminei, poria and polygala. It’s main effect lies on developing the effect of memorizing,as well as invigorating vital energy to educate heart、 tranquilizing and sedating the mind、expectorant、and other effects. Kaixinsan is mainly used for the treatment of deficiency of heart-qi,emotional restless, insomnia forgetful, which can change the symptoms of Patients with senile dementia and make them to be better, as well as pathological changes in brain physiology of animal models.Using single factor and orthogonal experiment to take a preliminary study about the alcohol extraction of Kaixinsan.By setting four factors:the amount of ethanol、ethanol concentration、extraction time and extraction times,evaluating through the yield of dry cream and the content of total saponins, combined with the actual needs of the extraction process and production,I found a better alcohol extraction process: extracting 2 times,each time lasts 96 h and using 75% ethanol by the amount of 7 folds of the amount of Kaixinsan.Using the SRB method to have a preliminary study about the possible effect of alcohol extracts on nerve cells,it indicated that Kaixinsan alcohol extract has no toxic effect on N2 A cells.Using solvent method to get the ingredient of chloroform soluable, ethyl acetate soluable, n-butanol soluable and water soluable of Kaixinsan.Besides, SRB method is used to do a study of biological activity on different extracting parts. The results show that the ingredient of chloroform soluable, ethyl acetate soluable have a strong inhibiting effect on N2 A cells,chloroform and ethyl acetate parts of N2 A cell inhibition rate take on certain concentration-response relationship with aging.N-butyl alcohol and water of Kaixinsan have a certain effect on promoting proliferation of N2 A cells.The SRB method is adopted to investigate different concentrations of n-butyl alcohol site in N2 A cells, the research results show that n-butyl alcohol part of Kaixinsan has certain effect on promoting proliferation of N2 A cells in 24 h after dosing; it has no significantly affect on N2 A cell inhibition rate in 48 h after dosing;and it has certain inhibition on N2 A cells in 72 h after the treatment.Presumably n-butanol part contains certain nerve cells proliferation of material, the active components of the prevention and treatment of AD, but does not contain nutrients.The SRB method is adopted to investigate different concentrations of water area in N2 A cells, the research results show that the water of Kaixinsan has certain effect on promoting proliferation of N2 A cells, it has no effect on promoting nerve cell proliferation without apparent attenuation within 72 h.Presumably water area contains certain promoting nerve cells proliferation material which can provide the energy and nutrients.Basing on the preliminary discussion of above, the alcohol of Kaixinsan effective parts of the prevention and treatment of AD are probably the alcohol extract and water area of Kaixinsan n-butyl alcohol parts.