Objective:This paper in fructus schisandrae related components of the extraction process, chemical composition and biological activity.Methods:By using solvent extraction and supercritical CO2extraction of fructus schisandrae related compounds of extraction and separation, get fat oil, always fructus schisandrae lignans and polysaccharides, on the basis of the best fructus schisandrae fat oil technology screening; With thin layer chromatography, high performance liquid chromatography and GC-MS fructus schisandrae chemical composition qualitative and quantitative analysis. Using the method of polysaccharide removal Fenton fructus schisandrae hydroxyl radicals activity determination of analysis.Results:The solvent extraction methods to determine the optimal process for three times the amount with petroleum ether extract1.2h, asking the fatty oil content is27.11%, the total wood grain of fructus schisandrae ester extract content is28.16%, polysaccharides extract content is25.58%. Supercritical CO2extraction method is used to determine the best technology of pressure for extraction30MPa, extracting temperature40℃, CO2flow15L/h, extraction time1.5h, asking the fatty oil content is28.54%. TLC good results, determine the total wood, fructus schisandrae ester extract contains fructus schisandrae ester armour, HPLC method of fructus schisandrae ester armour for the content determination, the results of the total wood ester, fructus schisandrae fructus schisandrae ester armour content is0.03%. GC-MS fat oil analysis to phthalic acid methyl ester, nutmeg acid methyl ester, phthalic acid ethyl ester, stearic acid methyl ester,17(acid methyl ester, and hexadecanoic acid methyl ester, linoleic acid methyl ester,9-18carbon ene acid methyl ester containing the6.32%,2.85%,3.07%,5.05%,9.63%,12.35%,45.19%,15.54%. Majority of fatty acid, linoleic acid content of the highest. Fenton method is used to determine the fructus schisandrae polysaccharides have antioxidant activity.Conclusion:In this paper the experimental study of fructus schisandrae related components were the best extraction process on the quality assessment, and the anti-oxidant activity polysaccharide. Therefore, in this paper the comprehensive exploitation and utilization of fructus schisandrae laid a good foundation. |