The Extraction Process Of Effective Parts In Onion And Biological Activity Research

Objective: To determine the optimum extraction process of flavonoids,polysaccharides and volatile oil respectively in onion,and to study the antioxidant and antibacterial activities of the extracts,what was to lay the foundation for further development and utilization of onion and its preparation.Methods: To optimize the extraction process of flavonoids by orthogonal design method,that index is comprehensive rate incluced spiraeoside,rutin,quercetin;The content of polysaccharide in onion was determined by phenol sulfuric acid method and the optimum extraction process was determined;The best extraction method was obtained by using the yield of onion volatile oil.The antioxidant activity of each extract was studied by DPPH and three phenol method and K-B method and MIC method were used to study the antibacterial activity of extracts.Results: The optimum extraction conditions of the flavonoids and polysaccharides were determined,what respectively were ethanol concentration 80%,material liquid ratio 1:30,extraction time 4d and extraction time was 5h,the ratio of material to liquid was 1:25,the extraction temperature was 70?,and the concentration of alcohol was 80%.The best extraction method of volatile oil is ethanol dehydration and dehydration.It was confirmed that the antioxidant activity of onion extracts,what was flavonoids>volatile oil >onion extract Commercially available 1 > onion extract Commercially available 2 >polysaccharides.It was confirmed that the onion flavonoids were sensitive to MASA,and its MIC was determined to be 8.514 mg/m L,MBC was 17.028 mg/m L.Conclusion: The results of multi component extraction of onion laid the foundation for the establishment of quality standard.The results of antioxidant activity and antibacterial activity of the extract were provided as the basis for the development of functional food and new antimicrobial agents.