The Construction And Evalution Of TCM Syndrome Model Of Dampness Stagnancy And Spleen Deficiency Induced By Compound Factors In Rats

Objective: To induce the TCM syndrome model of dampness stagnancy and spleen deficiency in rats and to evaluate that if the model complying with the TCM theory, in order to provide a reliable model for research on effect and mechanism of Chinese medicinal herbs with function of alleviating water retention and attributing spleen meridian.Methods: Multi factors as high-fat and low-protein diet and swimming to over-fatigue were given for long time to the rats to induce TCM syndrome Model of dampness stagnancy and spleen deficiency. The general signs, autonomic activities, metabolic, gastrointestinal functions, immune functions, blood routine examination, liver functions, water-dampness conditions were observed, and then the model was evaluated with PLS.Results: Compared with that in the control group, the body weight, autonomic activities, metabolic activities, gastrointestinal functions and immune functions in model group decreased with significant differences. The general signs, blood routine examination, liver functions, and water-dampness conditions also showed the variation tendency in different degrees. The signs inosculated with TCM syndrome model of dampness stagnancy and spleen deficiency and confirmed that the induction of the model is successful. The evaluation system was proved conforming to the standard of TCM syndrome model of dampness stagnancy and spleen deficiency and provided the scientific evidence for the model of induction.Conclusion: The TCM syndrome model of dampness stagnancy and spleen deficiency can be induced successfully with the compound factors in rats. Evaluation of this model proved that the model is reasonable and reliable.