Preparation Of Law Eucommia Leaf Accumulation Of Active Ingredients And Extracts

Eucommia(Eucommia ulmoides Oliv) for our unique Eucommia Ke Du Zhong genus, is a valuable tonic herbs. Modern research has found Eucommia leaf also has a high medicinal value, Eucommia leaves and skins are now contained in a(first edition 2010), "Chinese Pharmacopoeia", with strong bones, liver and kidney, and miscarriage and other effects.Medicinal active ingredients is an important indicator of the quality of the merits of traditional Chinese medicine, the content of eucommia leaves at different times of the active ingredients will change, and the growing season, harvest time, weather conditions and other factors will produce different effects on the active ingredient content Learn Eucommia leaf variation in different stages of active ingredients and timely harvest is to protect and improve the yield and quality of key medicines.Leaf extract is extracted from the leaves by step gutta prepared from refining, etc., because the gutta variety, origin, light, soil, field management, harvesting and processing, processing and other factors, gutta quality are affected. In order to obtain high-quality leaf herbs Eucommia, Eucommia leaf paper four kinds of active ingredient as an index to study the accumulation rule eucommia leaves the main active ingredient, to find the best harvest period for preparation and quality control method leaf extract were the study. By 3 batches were prepared in a pilot leaf extract.Results of this study are as follows:An established method for the determination by HPLC Eucommia leaves the active ingredient, the optimum chromatographic conditions: Column: Thermo Hypersil Gold C18(250mm × 4.6mm × 5 μm); methanol as mobile phase A, to 0.5% an aqueous solution of phosphoric acid as mobile phase A gradient elution; flow rate: 1.0mL / min;( 0~30 min,A:5%�'10%,B:95%�'90%;30~70 min,A:10%�'25%,B:90% �'75%); detection wavelength was 206 nm(0 ~ 15 min), 236 nm(15 ~ 55 min); the volume flow rate 1.0 mL / min. The results show different picking period of Eucommia leaves Aucubin, chlorogenic acid, the car leaves asiatica, content difference geniposidic acid is large, the best harvest terms of chlorogenic acid as harvest index, the Eucommia leaf period of the end of May to early June; terms of iridoid class(aucubin, geniposidic acid, car leaves asiatica) for harvest index, the best Eucommia leaf harvest for the end of May to 6 in early May; Eucommia leaf terms of comprehensive and effective ingredient for harvest index, so gutta optimum leaf harvest is the end of May to early June.Second, the preferred material conditions leaf extract prepared Eucommia leaf extract flash extraction method, spray drying method were leaf extract, Eucommia leaves determination of the active ingredient by high performance liquid chromatography. The results show that extracts Aucubin, chlorogenic acid, car asiatica leaf content geniposidic acid about four times higher than the Eucommia leaf.Third, simple purification of the active ingredient in Eucommia leaf: Using HPD100 macroporous resin adsorption, with distilled water and 50% ethanol, a flow rate of 2.0mL / min of elution, water will collect the red liquid solution and ethanol measured Aucubin chlorogenic acid, car asiatica leaf content geniposidic acids. The results showed that leaf extract had post-column content than before through the column was significantly increased to 2-4 times.