Model Establishment And Evaluation Of Retained Fluid In Upper-jiao

Object: To explore rat model establish of retained fluid in Upper‐jiao that accords with Traditional Chinese Medicine syndrome characteristics and then to carry out biological characteristics study.Methods: We randomly divided 40 rats into control group and model group, each of 20.All rats of both groups fed ad libitum. The model group was given abdominal subcutaneous injection of isoprenaline(ISO) 20mg/kg in D1, 10mg/kg in D2, 5mg/kg in D3 and 3mg/kg from D4 on, once per day for 20 consecutive days and followed by 2‐week observation; while the control group was given abdominal subcutaneous injection of equivalent NS. In D34 we operated tracheal intubation on the model group and carried out cold stimulation for 7 days after the surgery. Then we observed general conditions of the rats and detected lung permeability index, Bronchoalveolar lavage fluid pumping back quantity,lung wet‐and‐dry ratio, weight of heart and lung as well as organ index, the expression of creatine kinase(CK) and took histopathological examination.Results: Compared to the control group, the rats of the model group had significantly increased lung permeability index(P<0.05), Bronchoalveolar lavage fluid pumping back quantity(P<0.05), lung wet‐and‐dry ratio(P<0.05), heart weight and organ index(P<0.01) and CK expression(P<0.05) and lung weight and organ index tended to increase. In addition, HE staining of the model group showed abnormal alveolar wall structure, less alveolus, most alveolus filled with homogeneous pink protein liquid, loose and disorderedcardiac muscle fibers and hypertrophy of cardiocytes with eosinophilic degeneration and(or) necrosis of part of cardiocytes.Conclusion: Animal model of retained fluid in Upper‐jiao has been preliminarily established.