The Dynamic Expression Of AQP1, Muc5ac In Chronic Obstructive Pulmonary Disease Model Rats Of Syndrome Of Accumulation Of Cold Fluid In The Lung In Lung Tissue

Objective: According to the previous basic research on chronic obstructivepulmonary disease (chronic obstructive pulmonary disease, COPD) and accumulation ofcold fluid in the lung syndrome theory, with AQP1water channel protein in lung tissue andmucin Muc5ac as the starting point,to further explore the COPD accumulation of cold fluidin the lung disease machine dynamic evolution and water channel AQP1and mucinMuc5ac relationship, in order to enrich the theory of accumulation of cold fluid in the lung.It continues by cold stimulation combined with smoking and intratracheal instillation oflipopolysaccharide (LPS) rat model with the method of COPD accumulation of cold fluidin the lung syndrome, evolution of pathogenesis of budesonide by observing the model rats60days of natural evolution and the warming drink, intervention, explains COPD colddrink lung syndrome model of rat lung tissue water channel protein AQP1, and mucinMuc5ac and pulmonary function, the corresponding lung histomorphology changes (10days for a stage), as well as the warming drink fabric water channel protein AQP1, andmucin Muc5ac role in the regulation of COPD accumulation of cold fluid in the lung ratmodel of Lung Group, determine the target and aging with warming drink side, to providenew ideas and theoretical support for the prevention and treatment of diseases, todetermine the guiding preventive measures and treatment timing and duration.Methods: The model with the COPD syndrome of cold fluid retention in lungdisease copied out by smoking and intratracheal instillation of LPS accompanied by coldstimulation (0℃refrigerator freezer, ice and water mixture drinking),dynamicobserve(interval of10days,60days) the rat model of the natural state of evolution, and the disease model for dynamic intervention with warming drink and budesonide, observe thegeneral model of rat, pulmonary pathology, lung function testing related indicators, AQP1water channel protein in lung tissue, mucin Muc5ac.Results: This program use preliminary normative to copy out the COPDaccumulation of cold fluid in the lung of rats model. Index form and behavior in model ratsof change and COPD accumulation of cold fluid in the lung syndrome are reliable toclinical manifestations. After modeling the inspiratory resistance and expiratory resistancerats increased, decreased lung compliance, water channel protein decreased expression ofAQP1in lung tissue, Muc5ac expression increased, pathology showed lung tissue changesin inflammatory injury and fibrosis. On the60day after removal of molding factors, alongwith the prolonged duration of symptoms and indexes of the model rats had no obviousimprovement, and deteriorated in40days or so, the weakening of accumulation of coldfluid in the lung syndrome, is transformed into the other card type trend. Rat model ofsymptoms by warming drink party intervention gradually improved, improve lung function,water channel protein AQP1expression increased, Muc5ac expression decreased, thepathological sections showed inflammatory injury was relieved, reached the peak at40days, close to the normal level in rats. Rat model of budesonide intervention symptomsalso improved, but the water channel protein AQP1expression did not increase remarkably,mucin Muc5ac has no obvious fdecrease, the pathological sections showed decreasedinflammatory injury.Conclusion: Cold stimulation combined with smoking and intratracheal instillation ofLPS is the establishment of animal model combining with clinical practical ideals diseasesof COPD accumulation of cold fluid in the lung of rats model of has a good reliability andstability. This is critical for the formation of COPD accumulation of cold fluid in the lungsyndrome pathogenesis evolution, reduced expression of water channel protein AQP1,mucin Muc5ac expression increased with the close relationship between the formation ofphlegm. Accumulation of cold fluid in the lung syndrome might be COPD early or acutesyndromes, with warming drink to the expression of AQP1water channel protein in lungtissue up-regulation and down-regulation of the expression of mucin Muc5ac, eliminatingphlegm, has a definite effect on COPD accumulation of cold fluid in the lung syndrome,and treatment of the disease in aging water channel protein, AQP1, Muc5ac mucin is oneof its targets.