The Study Of Speripheral Blood Plasma And Red Blood Cells Alpha-synuclein Oligomers Level In Parkinson Disease

Objective At present,Parkinson disease has become the second largest degenerative diseases of nervous system.But,there is still no effective method for diagnosis,especially laboratory indicators,and it still lack of early diagnosis method.Alpha-synuclein is the main constituent of Louis bodies,and it abnormality aggregation can lead to dopamine neurons apoptosis or death,and the concentration α-syn in cerebrospinal fluid and peripheral blood have increased abnormally.To detect the patient of Parkinson disease peripheral blood red cells and plasma α-syn oligomer,and with the course,H-Y grade,UPDRSⅢ scores,NMSQuest scores,MMSE scores analyzed by correlation,and hope that α-syn oligomers in the peripheral blood red blood cells and plasma can be improve the level of clinical diagnosis and intervention.Methods Selecting 30 patients of Parkinson disease admitted in department of neurology and the general hospital of Dalian Friendship Hospital,from March 2013 to December 2014,as PD group,and exclude the condary Parkinson disease,Alzheimer’s disease,Louis body dementia,Acute cerebrovascular disease.Selecting 30 cases of healthy physical examinees as control group.PD group evaluates UPDRSⅢ scale,NMSQuest scale,MMSE scale.The level of α-syn oligomer in peripheral blood red cells and plasma were detected by ELISA method.Statistical analyses were performed using SPSS17.0.Results 1.The level of α-syn oligomer in peripheral blood plasma of PD group(111.22±66.23pg·m L-1) were significantly higher than that of the controlgroup(77.86±39.20pg·m L-1),the differences were statistically significant(t=2.35,P=0.02,P<0.05).2.The level of α-syn oligomer in peripheral blood red cells of PD group(177.42±72.20pg·m L-1) were significantly higher than that of the control group(97.43±36.13pg·m L-1),the differences were statistically significant(t=5.4,P=0.00,P<0.01).3.The α-syn oligomer in PD patients peripheral blood red cells were significantly higher than that in peripheral plasma,and the differences were statistically significant(t=3.66;P<0.05).The α-syn oligomer in control group patients peripheral blood red cells were significantly higher than that in peripheral plasma,and the differences were statistically significant(t=2.24;P<0.05).4.The level of α-syn oligomer in peripheral plasma of PD group was not relevant to course,H-Y grade,UPDRSⅢ,NMSQuest,MMSE(r=0.33,0.18,0.25,0.03,0.003;P>0.05).5.The level ofα-syn oligomer in peripheral blood red cells of PD group was not relevant to course,H-Y grade,UPDRSⅢ,NMSQuest,MMSE(r=-0.11,0.08,0.22,-0.13,0.15;P>0.05).Conclusion 1.The α-syn oligomer in PD patients peripheral blood red cells were significantly higher than that in peripheral plasma,so the red blood cells are more sensitive than plasma,so it can be as one of the methods for diagnosis of Parkinson disease.2.α-syn oligomer in peripheral blood red cells and plasma of Parkinson disease were not relevant to course,H-Y grade,UPDRSⅢ scores, NMSQuest scores and MMSE scores,so it can’t monitor the disease progression.