Alpha—synuclein Oligomer In Plasma And Saliva As A Biomarkerfor Parkinson's Disease

Background and ObejectsParkinson's disease(PD)is a common neurodegenerative disease in middle-aged and elderly people.Clinically,resting tremor,bradykinesia,muscle rigidity and gait abnormalities as the main performance.The main pathological changes of PD are neurodegenerative diseases with the deletion of dopaminergic neurons in substantia nigra of the midbrain and the presence of Lewy bodies in substantia nigra neurons as the main pathological changes.Lewy bodies is an important diagnostic feature of PD,which contains a variety of components,and related studies have shown that ?-synuclein is the first confirmed by the PD-related protein is an important component of Lewy bodies,?-Synuclein is closely related to the formation of Lewy bodies and the occurrence of PD.There is growing evidence that aberrant ?-synuclein(?-Syn)aggregation is a key event in the pathological cascade of PD.The available data show that the abnormal expression and aggregation of ?-synuclein is an important part of the pathogenesis of PD.Previous studies mainly focused on cerebrospinal fluid,inconvenient drawing and a greater impact.Blood and saliva are more accessible and less damaging,making them an ideal biomarker for the detection of this marker.So this time our research focused on blood and saliva.In this paper,we examined the concentration of ?-synuclein in blood and saliva of patients with Parkinson's disease and control group to study their association with Parkinson's disease,Nuclear protein can be used as a peripheral biological marker of Parkinson's disease.MethodsCollecting from October 2015 to August 2017 35 cases of Parkinson's disease outpatient department or admission neurological outpatient department of Anhui Provincial Medical University Affiliated Provincial Hospital,25 cases of normal(control group)from the Provincial Medical Center of Anhui Medical University Medical Center.The blood and saliva were collected from patients with Parkinson's disease and healthy controls.The levels of ?-synuclein in blood and saliva of PD group and control group were detected to analyze the relationship between ?-synuclein level and severity of disease,duration of disease,Age and PD assessment scale motor score(score)of the correlation.ResultsThe level of ?-synuclein in plasma of PD group was higher than that of healthy control group.The level of ?-synuclein in saliva was lower than that in healthy control group(P<0.05).The difference was statistically significant.The level of plasma ?-synuclein in PD group was significantly higher than that in the control group(r =-0.025,P = 0.888),Hohen Yahr classification(r =-0.163,P = 0.348)There was no correlation between the scale of assessment scale(r =-0.159,P = 0.362)and age of onset(r =-0.034,P = 0.848).PD group salivary ?-synuclein content and course(r=-0.78,P=0.655),Hohen Yahr classification(r=-0.052,P=0.766),uniform PD rating scale exercise score(r=-0.182,P=0.295)was not correlated,and was negatively correlated with age(r=-0.425,P=0.011),age of onset(r=--0.336,P=0.048).The area under the ROC curve of plasma ?-synuclein as a PD marker in the diagnosis of PD was 0.727(P = 0.003).The optimal cutoff value was 157.86 ng / L,the sensitivity was 68.60% and the specificity was 72.00%.The salivary ?-synuclein as a diagnostic indicator,the area under the ROC curve was 0.785(P = 0.00),the best cut-off value was 211.14 ng / L,the sensitivity was 82.9% and the specificity was 80.0%.ConclusionThe sensitivity,specificity,and positive predictive value of salivary ?-synuclein in the diagnosis of PD are higher than those in plasma ?-synuclein,and may be more suitable as indicators for the diagnosis of PD.This study found that age,age of onset was negatively correlated with PD salivary ?-synuclein,which may be related to the lack of secretion of salivary glands after atrophy.