The Expression And Clinical Significance Of Twist And KiSS-1in Squamous Cervical Cancer

Objective: To study the expression of Twist and KiSS-1in thenormal cervical epithelium(NCE), cervical intraepithelial neoplasia(CIN) andsquamous carcinomas of cervix(SCC), and to investigate the relationship ofTwist and KiSS-1in the occurrence and development of squamous carcinomasof cervix. Methods: Immunohistochemistry Envision was employed to detectthe expression of Twist and KiSS-1in47cases of SCC,20cases of CIN and12cases of NCE. This study was to explore the relationship between the two kindsof protein expression and the clinical pathological parameters of SCC. χ2test orFisher’s exact test was used to identify the difference among groups forfrequency data; spearman correlation was conducted for correlation test.Kaplan-Meier and Log-rank test were used to detect the survival time and Coxproportional hazard analysis used for multivariable factors of SCC. Results:1.It was found that Twist was chiefly localized in the nucleus of NCE and CINbut mainly localized in cytoplasm of SCC. The positive expression rates ofTwist were escalated for16.67%（2/12）、30%（6/20）、85.11%（40/47）inNCE,CIN and SCC respectively. The positive expression rates of twist in SCCwere significantly higher than that in NCE and CIN. There had been significantdifferences in the positive expression rates of twist in SCC and NCE, so had inSCC and CIN. The positive expression rates of Twist in SCC were notassociated with clinical pathology index of SCC such as age, FIGO stages,historical differentiation, the depth of tumor invasion and lymph nodemetastasis, but there was a higher positive Twist expression tendency in lymphnode metastasis than not.2. KiSS-1was mainly localized in cytoplasm and cellmembranes, partly expressed in nucleus of different cervical tissues. The positive expression rates of KiSS-1were decreased with91.67%（11/12）、70.00%（14/20）、6.38%（3/47）in NCE,CIN and SCC respectively. The positiveexpression rates of KiSS-1in SCC were significantly lower than that in NCEand CIN. There had been significant differences in the positive expression ratesof KiSS-1in SCC and NCE, so had in SCC and CIN. The positive expressionrates of KiSS-1in SCC were also not associated with clinical pathologicalindex of SCC such as age, FIGO stages, historical differentiation, the depth oftumor invasion and lymph node metastasis.3. The expression of twist was notcorrelated with that of KiSS-1in SCC.4. Univariate analysis and Multivariateanalysis showed that the expression of Twist and KiSS-1were not correlatedwith the survival rate of cervical squamous cell carcinoma. Conclusion:1.Theexpression of Twist was elevated in normal cervical tissue, CIN and SCC,which may indicate the correlation during the initiation and development ofSCC. There was no correlation between the expression of Twist and thepathoclinical parameters, including the age, FIGO stages, historicaldifferentiation, the depth of tumor invasion and lymph node metastasis in SCC.2. The expression of KiSS-1was decreased, which indicated that they may becorrelated with the occurrence and development in SCC. There was nocorrelation between the expression of KiSS-1and the pathoclinical parameters,including the age, FIGO stages, historical differentiation, the depth of tumorinvasion and lymph node metastasis in SCC.3. The expression of Twist andKiSS-1in SCC were not associated with the prognosis of SCC patients.4.There was no correlation between Twist and KiSS-1expressed in SCC.