The Expression And Significance Of GRIM-19Protein In Cervical Squamous Cell Carcinoma

Objective: Objective to grope for the expression of GRIM-19protein incervical squamous cell carcinoma and its relationship with clinicalpathological characteristics and prognosis of patients, and provide referencebasis for individualized treatment for clinical.Methods:29cases of cervical squamous cell carcinoma were selectedfrom the fourth hospital of Hebei Medical University between January1,2008to December31,2008. According to the2009FIGO classification:3cases ofIa1stage, Ia2stage3cases, Ib1in4cases,5cases of Ib2stage, IIa1stage6cases, IIa2stage4cases, IIb4cases. The age of patients ranged from38to64years, average age is48years old. According to the FIGO histological grading:Grade1in0case,2grade13cases,16cases of grade3. The29cervicalcancer patients are as follows:①It was carried by2subtropical high andabove the title of doctor after gynecological examination;②The clinical data(including pathological data) was complete;③The follow-up data wasstandardized and systemic. All included patients were followed up by phone.A follow-up was started from completion of all the treatments, at the end ofDecember31,2013, the last follow-up covering all subjects. Select10patientswith myoma of uterus hysterectomy for patients with non cancerous cervicaltissues during the same period as normal controls (age40-62years, meanage48.5years). Immunohistochemical method was used to detect theexpression of GRIM-19protein in29cases of in cervical squamous cellcancer tissues resected by operation and10cases of normal cervicaltissue.Using Fisher’s exact test and chi square test for non parametric analysis,the expression level of GRIM-19protein in different clinical and pathologicalparameters of cervical squamous cell carcinoma; Kaplan-Meier and log-rank test was used for univariate survival analysis, comparison of the prognosis ofpatients with cervical squamous cell cancer with different expression levels ofGRIM-19protein (negative, weak positive, moderate positive, strong positivegroup); Using the multi factors Cox regression model for the analysis of thefactors affecting the prognosis of patients with cervical squamous cell cancer.Results: Highly expression of GRIM-19in normal cervix tissue, whilethe low expression or no expression in uterine cervical squamous cellcarcinoma tissue, cervical squamous cell carcinoma and normal cervicaltissues compared to the expression of GRIM-19protein have significantdifference (P<0.05), there was statistical significance. Nonparametric analysisshowed the level of GRIM-19protein expression was significantly correlatedwith cervical squamous cell cancer patients with clinical staging, andhistological differentiation(P=0.007、 P=0.026). But the expression ofGRIM-19protein was not correlated with cervical cancer patients’ age, tumordifferentiation,pelvic lymph node metastasis, primary tumor maximumdiameter (P>0.05). A total of7patients died during the follow-up period, totalsurvival time of the patients was8-71months, the median overall survivaltime was65months. The patients of the four groups (the protein expression ofGRIM-19was negative (-), weak positive (+), moderate positive (++), strongpositive (+++) groups) were respectively6,1,13,9cases, univariate survivalanalysis showed that, the total survival time were compared among the4groups, the difference was statistically significant (P=0.001). Cox regressionanalysis showed, expression of GRIM-19protein level(P=0.333) was not anindependent prognostic factor of patients with cervical squamous cell cancer.Conclusion: The expression of GRIM-19protein in cervical squamouscell carcinoma tissues is significantly reduced compared with normal cervicaltissues.The expression of GRIM-19is obviously correlated with clinicalstaging, and histological differentiation.There are significant differences ofoverall survival time in the four groups(GRIM-19negative,weakpositive,moderate positive,strong positive).It is known that the expressionlevel of GRIM-19is a significant prognostic factor in patients with cervical squamous cell carcinoma,but is not an independent prognostic factor inpatients with cervical squamous cell carcinoma.