| Objective. To summarize the features of clinicopathology, postoperative therapy andsurvival of the gastric cancer patients after radical operation. We detected thecorrelation of the patients’ clinicopathology and therapy with their survival. In themeanwhile, we tested the expression of LOX, VEGF-A, HIF1α and PDGFR-β in thetissues of gastric cancer and their clinical significance. Finally, we searched themolecular markers that could predict the risks of recurrence and metastasis, andsurvival prognosis.Methods. We did retrospective analysis of the gastric cancer patients who receivedradical operation and had complete follow-up information in PLA General Hospitalfrom January2007to June2008and statistical analysis the correlation of the patients’clinicopathology and postoperative therapy with their survival. We made tissuemicroarray from the patients’pathological specimens and detected the expression ofLOX, VEGF-A,HIF1α and PDGFR-β by tissue microarray and immunohistochemistry.We analyzed the relationship of the above factors’ expression and features ofclinicopathology and survival of the gastric cancer patients after radical operationwith SPSS19.0software.Results.1. The median follow-up time of the166gastric cancer cases was64.3months. Up to the end of follow-up (September1st,2013), there were110cases wasrecurrence or metastasis (66.3%) and101cases died (60.8%),the median DFS was25.4months, the median OS was35.2months, the5years’ disease free survival ratewas31.0%, and5-year overall survival rate was29.0%. Multiple factors analysis ofsurvival indicated that surgical margins, Borrmann types, TNM stages and adjuvantchemotherapy cycles are independent prognostic factors that affect the median DFS. While Borrmann types, TNM stages and adjuvant chemotherapy cycles areindependent prognostic factors that affect the median OS.2. The over expression rate of LOX in the tissues of gastric cancer (39.8％) wassignificantly higher than that in non-cancerous adjacent gastric tissue (18.6%). ThemDFS and mOS in the weak-expression group of LOX were significantly longer thanthe overexpression group.There was significant difference between them (mDFS:31.3VS15.1, P=0.005; mOS:49.2VS21.9, P=0.004).3. The over expression rate of VEGF-A in the tissues of gastric cancer (48.2％)was significantly higher than that in non-cancerous adjacent gastric tissue (14.6%).There was significant correlation between the expression of VEGF-A andtumour location(P=0.001).The mDFS and mOS in the weak-expression group ofVEGF-A were significantly longer than the overexpression group.There wassignificant difference between them (mDFS:31.3VS17.5, P=0.019; mOS:50.2VS26.4, P=0.034).4. The over expression rate of HIF1α in the tissues of gastric cancer (28.3%) wassignificantly higher than that in non-cancerous adjacent gastric tissue (12.6%). Therewas significant correlation between the expression of HIF1α, Borrmann types and thenumber of lymph node metastasis (P=0.024, P=0.025).The mDFS and mOS in theweak-expression group of HIF1α were significantly longer than the overexpressiongroup.There was significant difference between them (mDFS:27.0VS16.1, P=0.048;mOS:39.8VS20.4,P=0.046).5. The over expression rate of PDGFR-β in the tissues of gastric cancer (33.1%)was significantly higher than that in non-cancerous adjacent gastric tissue (6.0%).There was significant correlation between the expression of PDGFR-β, thehistological classification, the number of lymph node metastasi and TNM stage.(P=0.021, P=0.029, P=0.004).The mDFS and mOS in the weak-expression group ofPDGFR-β were significantly longer than the overexpression group.There was significant difference between them (mDFS:30.4VS15.1, P=0.040; mOS:46.8VS22.5, P=0.007).6. There was significant correlation between the expression of LOX, VEGF-A,HIF1α and PDGFR-β in the tissues of gastric cancer.7. The COX proportional regression analysis showed that the overexpression ofLOX was an independent risk factors in predicting mDFS and mOS.8. mDFS and mOS of the patients who had LOX, VEGF-A, HIF1α and PDGFR-βco-overexpression were significant shorter than those who had three, two or one ofthe above factors overexpressed (mDFS:P＜0.05, mOS:P＜0.05).Conclusion. Surgery margins, Bormann types, TNM stages, adjuvant chemotherapycycles and the expression of LOX were independent prognostic factors that affectDFS. Borrmann types, TNM stages, adjuvant chemotherapy cycles and the expressionof LOX were independent prognostic factors that affect OS. |
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